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  1. LEAKING SAMPLES DURING TRANSPORT: If any samples are found to be leaking during transport, the leak should be contained/absorbed by the packaging. Model rules produced by the Pathology Department must be followed by all couriers and covers what to do in the event of a leaking sample-Excerpt from the Model Rules for Porters

  2. A sample leaks in transit. A sample is dropped and breaks in transit. A sample is dropped and its loss is not noticed. Instructions to reduce additional risk: To minimise the chance of dropping specimens, carry all specimens in a pathology collection trolley, or transport box, or plastic carrier bag, do not carry by hand or place in a pocket.

    • Summary List For Sample Rejection
    • Quality Assurance
    • Department-Specific
    Incorrect sample types received:
    Samples in incorrect containers (e.g. cervical cytology must be a ThinPrep vial; urine cytology must be in a uricyte container).
    Insufficient sample received.
    No sample received.
    Sample contamination (e.g. being in the same bag as a leaking sample).
    Samples are high risk or infectious.
    Samples that are received in expired tubes.
    Sample Receptionwill not accept samples packaged with needles of any kind.
    Haematologycannot accept frozen whole blood for testing.
    Coagulationcannot accept over or under filled samples for testing.
    Coagulationcannot accept previously frozen samples that have thawed in transit.
  3. The sample will be stored for a period of time allowing the requestor to contact the department (Consultant Microbiologist). As soon as requester is aware they require a repeat test or additional tests on the sample, they should contact the laboratory as the specimen may have a short period of time in the lab before being discarded.

  4. Sample Doesn't Sink to the Bottom of the Well. Sample Leaking Out of Well. Bands are Smeared Vertically. Too Many Bands. Gel Running Unusually Slowly. Gel Running Unusually Fast. Protein Bands Too Close Together (Not Completely Resolved) Leftmost and/or Rightmost Bands of the Gel are Distorted. Bands Form Smiling Shapes.

  5. What should I do? Occasionally the laboratories are unable to test samples sent to them. This may be because the amount collected is insufficient for full analysis, the sample has deteriorated or clotted or it has haemolysed (where red blood cells damaged during sample collection leak their contents into the surrounding plasma).

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  7. There are five different signs that indicate that there are problems with SDS-PAGE sample preparation: sample leaking out of the well during or after loading, samples clumping and not migrating, you aren’t seeing bands, you’re seeing too many bands, or you’re getting smeared bands. SDS-PAGE sample preparation issues: Samples leaking out ...

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