Including BCR-ABL Stable Cell Line - BaF3 and BCR-ABL-T315I Stable Cell Line - BaF3. Use in cell-based in vitro screening of drug candidates against chronic myeloid leukemia.
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A BCR-ABL test is most often used to diagnose or rule out chronic myeloid leukemia (CML) or a specific form of acute lymphoblastic leukemia (ALL) called Ph-positive ALL. Ph-positive means a Philadelphia chromosome was found. The test is not used to diagnose other types of leukemia.
The BCR-ABL test stands as a pivotal tool in the diagnosis and management of chronic myeloid leukemia. Its ability to pinpoint the presence of the BCR-ABL fusion gene offers insights into disease progression, guiding tailored treatment plans and fostering better patient outcomes.
Nov 15, 2013 · The mean BCR-ABL1/ABL1 ratio was 55.91% (range: 34.83-98.01%) at diagnosis, 9.66% (range: 6.72-13.53%) in the second group (6-14% values) and 0.093% (range: 0.05-0.18%) in the third group (MMR patients).
- Sarah Huet, Denis Souche, Jean-Pierre Magaud, Pascale Cony-Makhoul, Yann Guillermin, Mael Heiblig, M...
- 2013
- What is a BCR-ABL1 genetic test?
- What is it used for?
- Why do I need a BCR-ABL1 genetic test?
- What happens during a BCR-ABL1 genetic test?
- Will I need to do anything to prepare for the test?
- Are there any risks to the test?
- What do the results mean?
- Is there anything else I need to know about a BCR-ABL1 genetic test?
- References
- Related Health Topics
A BCR-ABL1 genetic test uses a sample of blood or bone marrow to look for an abnormal gene called BCR-ABL1. This abnormal gene is involved in certain types of blood cancer. It's found in:
•Most people who have chronic myeloid leukemia (CML), which is also called chronic granulocytic leukemia or chronic myeloid leukemia
•Some people who have certain types of acute lymphoblastic leukemia (ALL)
•Some people with acute myeloid leukemia (AML), but very rarely
The abnormal BCR-ABL1 gene is made when two separate genes merge together and become one gene. Genes are short sections of DNA. DNA is made of long strands of material that carry information that controls what you look like and how your body works. DNA is packaged into structures in your cells called chromosomes. Normally, most of your cells have the same set of 23 pairs of chromosomes.
The abnormal BCR-ABL1 gene is formed when pieces of chromosomes 9 and 22 break off and trade places. The broken piece of chromosome 9 includes part of the ABL1 gene. It attaches (fuses) to part of the BCR gene on chromosome 22. This makes the abnormal fusion gene, BCR-ABL1. The changed chromosome 22 is called a "Philadelphia chromosome" after the city where researchers discovered it.
BCR-ABL1 genetic testing is most often used with other tests to diagnose or rule out two specific types of blood cancer:
•Chronic myeloid leukemia (CML).
•Philadelphia-positive acute lymphoblastic leukemia (Ph+ ALL). This is a type of acute lymphoblastic leukemia that has the Philadelphia chromosome.
Certain types of BCR-ABL1 testing may also be used to help plan treatment for these blood cancers. There are several treatment options. Some medicines target the protein made by the BCR-ABL1 gene. In general, these types of medicines cause less harm to normal cells than cancer chemotherapy or radiation therapy do.
If your health care provider thinks you may have a type of leukemia that involves the BCR-ABL1 gene, you may need a BCR-ABL1 genetic test to make a diagnosis. Signs and symptoms of these blood cancers include:
•High levels of white blood cells on a complete blood count (CBC) test
•Fatigue
•Fever
•Weight loss
•Night sweats (heavy sweating during sleep)
A BCR-ABL1 genetic test usually uses a sample of blood or bone marrow.
If you are having a blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. You may feel a little sting when the needle goes in or out. This usually takes less than five minutes.
If you are having a bone marrow test, a provider usually takes a sample from the back of the hip bone. You will be given an injection (shot) of medicine to numb the area. You may also be given medicine to help you relax or sleep.
A bone marrow test has two parts that are usually done one after the other:
•For a bone marrow aspiration, the provider pushes a thin needle into the bone and removes a sample of bone marrow fluid with a syringe attached to the needle.
•For a bone marrow biopsy, the provider inserts a hollow needle into the bone and twists the needle to take out a small piece of bone marrow tissue. You may feel some pressure or brief pain while the sample is being taken.
You usually don't need any special preparations for a blood test. If you're having a bone marrow test, your provider will tell you whether you need to fast (not eat or drink) for a few hours before the procedure.
There is very little risk to having a blood test. You may have slight pain or bruising at the spot where the needle was put in, but most symptoms go away quickly.
After a bone marrow test, you may feel stiff or sore where the sample was taken. This usually goes away in a few days. If you need pain relief, talk with your provider.
If you had BCR-ABL1 genetic testing to diagnose a blood cancer, your provider will probably use the results of more than one test to make a diagnosis. Your provider will likely diagnose either chronic myeloid leukemia (CML) or Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) if your tests show all three of these results:
•Abnormal white blood cells
•The BCR-ABL1 gene
•The Philadelphia chromosome
In certain cases, a person may have CML without having the Philadelphia chromosome, but this isn't common.
If you had a BCR-ABL1 gene test to see if your cancer treatment is working, your provider will compare your results with past test results to look for changes over time. The way your results are reported depends on which test you had. Ask your provider to explain what your test results mean for your health and treatment plan. In general:
Labs use different methods to test for the BCR-ABL1 gene and Philadelphia chromosome. The test method can affect your results. So, it's important to have your tests done the same way, and usually in the same lab. This allows your provider to compare your results over time. If you have questions about how your tests are done, ask your provider.
1.American Cancer Society [Internet]. Atlanta: American Cancer Society Inc.; c2022. What Causes Chronic Myeloid Leukemia; [updated 2018 Jun 19; cited 2022 Aug 29]; [about 2 screens]. Available from: https://www.cancer.org/cancer/chronic-myeloid-leukemia/causes-risks-prevention/what-causes.html
2.Cancer.net [Internet]. Alexandria (VA): American Society of Clinical Oncology; c2005-2022. Leukemia: Chronic Myeloid: CML: Introduction; [updated 2018 Mar; cited 2022 Aug 29]; [about 2 screens]. Available from: https://www.cancer.net/cancer-types/leukemia-chronic-myeloid-cml/introduction
3.Leukemia and Lymphoma Society [Internet]. Rye Brook (NY): Leukemia and Lymphoma Society; c2015. Chronic Myeloid Leukemia; [cited 2022 Aug 29]; [about 3 screens]. Available from: http://www.lls.org/leukemia/chronic-myeloid-leukemia
4.Mayo Clinic [Internet]. Mayo Foundation for Medical Education and Research; c1998-2022. Bone marrow biopsy and aspiration: Overview;[cited 2022 Aug 29]; [about 8 screens]. Available from: https://www.mayoclinic.org/tests-procedures/bone-marrow-biopsy/about/pac-20393117
5.Mayo Clinic [Internet]. Mayo Foundation for Medical Education and Research; c1998-2018. Chronic myelogenous leukemia: Overview;[cited 2022 Aug 29]; [about 6 screens]. Available from: https://www.mayoclinic.org/diseases-conditions/chronic-myelogenous-leukemia/symptoms-causes/syc-20352417
6.Mayo Clinic: Mayo Medical Laboratories [Internet]. Mayo Foundation for Medical Education and Research; c1995-2022. Test ID: BADX: BCR/ABL1, Qualitative, Diagnostic Assay: Clinical and Interpretive; [cited 2022 Aug 9]; [about 2 screens]. Available from: https://www.mayocliniclabs.com/test-catalog/overview/89006#Clinical-and-Interpretive
Results from the RT-qPCR assays are reported as a ratio between BCR::ABL1 and ABL1. The major e13a2 and/or e14a2 transcripts are also reported on the international scale (IS) by use of a laboratory specific conversion factor.
Aug 18, 2021 · Testing for BCR-ABL detects the Ph chromosome (the derivative chromosome 22) and BCR-ABL fusion gene or its transcripts, the RNA copies made by the cell from the abnormal stretches of DNA. The presence of the BCR-ABL abnormality confirms the clinical diagnosis in CML or a type of ALL.
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The BCR-ABL1/ABL1 ratio obtained in the German laboratory among the patients who have achieved MMR was 0.12%, corresponding with the 3-log reduction from the standardized baseline established in the IRIS trial, fixed at 0.10% (MMR) .
