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Method of DNA sequencing
- Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase -specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides.
en.wikipedia.org/wiki/Maxam–Gilbert_sequencing
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Why did Walter Gilbert and Frederick Sanger get a Nobel Prize?
Mar 21, 2011 · In 1976, Allan Maxam and Walter Gilbert developed a method by which the ends of the DNA molecule could be marked using radioactive substances. After undergoing treatment with small amounts of chemicals that react with specific nucleotides, DNA fragments of varying lengths can be obtained.
- Interview
Interview with the 1980 Nobel Laureate in Chemistry, Walter...
- Nobel Lecture
Nobel Lecture - Walter Gilbert – Facts - NobelPrize.org
- Biographical
In the middle seventies, Allan Maxam and I developed the...
- Frederick Sanger
Frederick Sanger - Walter Gilbert – Facts - NobelPrize.org
- Paul Berg
Paul Berg - Walter Gilbert – Facts - NobelPrize.org
- Interview
Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase -specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides .
Maxam–Gilbert sequencing requires a small additional logical step: Ts and As can be directly inferred from a band in the pyrimidine or purine lanes respectively, while G and C are indicated by the presence of dual bands in the G and A + G lanes, or C and C + T lanes respectively.
- James M. Heather, Benjamin Chain
- 10.1016/j.ygeno.2015.11.003
- 2016
- Genomics. 2016 Jan; 107(1): 1-8.
Together with Allan Maxam, Gilbert developed a new DNA sequencing method, Maxam–Gilbert sequencing, [23] [24] using chemical methods developed by Andrei Mirzabekov. His approach to the first synthesis of insulin via recombinant DNA [ 25 ] lost out to Genentech 's approach which used genes built up from the nucleotides rather than from natural ...
- A Very Short History of Two DNA Sequencing Methods
- How Does Maxam–Gilbert Sequencing Work?
- Why Did It Lose Popularity?
- What Is Maxam–Gilbert Sequencing Used For Today?
- Usefulness in Obscurity: Summing It Up
- Reference
First, a bit of history. In the mid-1970s, two methods were developed to sequence DNA directly. These were: 1. The Maxam–Gilbert sequencing method 2. The Sanger chain-termination method In 1980, both Walter Gilbert and Frederick Sanger were awarded The Nobel Prize in Chemistry for contributing to the determination of base sequences in nucleic acids...
Maxam–Gilbert sequencing is also known as chemical sequencing because chemical reactions, rather than DNA and RNA amplification, are the basis of the method. It’s quite easy to understand, though, and proceeds in just 4 steps:
Maxam–Gilbert sequencing, although based on simple principles, came with a whole lot of trouble. Here are a few of its major drawbacks.
While the Maxam-Gilbert method is not used as much as it once was, it is still used in some specialized applications where aspects of the process (namely the chemical cleavage step) make it useful
And that’s a summary of Maxam–Gilbert sequencing. How it works, the newer and more powerful sequencing methods like next-generation sequencing that have consigned to relative obscurity, and some of its remaining applications. The somewhat tedious and hands-on chemical steps involved make it ideal for detecting modifications to and, indeed, the sequ...
Sidote DJ et al. (2008) Structure of the Staphylococcus aureus AgrA LytTR domain bound to DNA reveals a beta fold with an unusual mode of binding. Structure 16:727–35
In the middle seventies, Allan Maxam and I developed the rapid chemical DNA sequencing. At this time, I also became interested in and developed some of the recombinant DNA techniques, specifically showing that blunt end ligation was efficient in putting DNA fragments together.
Walter Gilbert is an American molecular biologist who was awarded a share (with Paul Berg and Frederick Sanger) of the Nobel Prize for Chemistry in 1980 for his development of a method for determining the sequence of nucleotide links in the chainlike molecules of nucleic acids (DNA and RNA).
