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      • If an agglutination reaction occurs, the red blood cells tested have that specific antigen—the A or B glycoprotein—on their surface. For example, if the anti-A serum is mixed with an individual’s red blood cells and an agglutination reaction occurs but does not when mixed with anti-B serum, that person has blood type A.
      www.osmosis.org/answers/agglutination
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  2. Feb 28, 2023 · The purpose of agglutination is to detect the presence of specific antigens or antibodies in samples of bodily fluids, such as blood, saliva, urine, or cerebrospinal fluid (CSF); as well as to identify specific strains of bacteria in microbiological cultures.

  3. Apr 21, 2024 · Agglutination can be used as an indicator of the presence of antibodies against bacteria or red blood cells. Agglutination assays are usually quick and easy to perform on a glass slide or microtiter plate (Figure \(\PageIndex{1}\)).

  4. Jul 30, 2023 · Agglutination: The binding of antibodies to multiple antigens on the surface of the pathogen leads to agglutination, causing the particles to clump together. This process immobilizes the pathogens, making it easier for other immune cells, such as phagocytes, to recognize and engulf them for destruction.

    • What Is agglutination?
    • Prozone Phenomenon
    • Types of Agglutination Reactions
    • Active Agglutination
    • Passive Agglutination
    • Hemagglutination Test
    • Applications of Agglutination Reactions
    • Further Readings

    Agglutination is the visible expression of the aggregation of antigens and antibodies. Agglutination reactions apply to particulate test antigens that have been conjugated to a carrier. The carrier could be artificial (such as latex or charcoal particles) or biological (such as red blood cells). These conjugated particles are reacted with patient s...

    The condition of excess antibody, however, is called a prozone phenomenon.At a high concentration of antibody, the number of epitopes are outnumbered by antigen-binding sites. This results in the univalent binding of antigen by antibody rather than multivalently and thus, interferes in the crosslinking of antigen (Lattice formation). Occasionally, ...

    Agglutination reactions can be broadly divided into three groups: 1. Active/Direct agglutination 2. Passive agglutination 3. Hemagglutination

    Agglutination reactions where the antigens are found naturally on a particle are known as direct agglutination. In active agglutination, direct agglutination of particulate antigen with specific antibody occurs. Direct bacterial agglutination uses whole pathogens as a source of antigen. It measures the antibody level produced by a host infected wit...

    Passive agglutination employs carrier particles that are coated with soluble antigens. In this either antibody or antigen is attached to certain inert carrier thereby, particles or cells gets agglutinated when corresponding antigen or antibody reacts. Latex particles, Carbon particles, Bantonite etc. are used as inert carriers. E.g. Antigens coated...

    RBCs are used as carrier particles in hemagglutination tests. RBCs of sheep, human, chick, etc. are commonly used in the test. When RBCs are coated with antigen to detect antibodies in the serum, the test is called indirect hemagglutination (IHA) test. Hemagglutination uses erythrocytes as the biological carriers of bacterial antigens, and purified...

    Cross-matching and grouping of blood.
    Identification of Bacteria. E.g. Serotyping of Vibrio cholera, Serotyping of SalmonellaTyphi and Paratyphi.
    Serological diagnosis of various diseases. E.g Rapid plasma regains (RPR) test for Syphilis, Antistreptolysin O (ASO)test for rheumatic fever.
    Detection of unknown antigen in various clinical specimens. E.g. detection of Vi antigen of SalmonellaTyphi in the urine.
    https://bio.libretexts.org/Bookshelves/Microbiology/Book%3A_Microbiology_(Boundless)/12%3A_Immunology_Applications/12.2%3A_Immunoassays_for_Disease/12.2E%3A__Agglutination_Reactions
    https://www.brainkart.com/article/Types-of-Agglutination-Reactions—Antigen-Antibody-Reactions_20188/
    http://www.biosciencenotes.com/agglutination/
  5. Apr 10, 2024 · Agglutination reaction is an antigen-antibody reaction that leads to the visible clumping of particles. It occurs when a particulate antigen combines with its corresponding antibody in the presence of electrolytes, at a specific temperature and pH.

  6. An agglutination reaction can be readily observed, usually after 10 to 15 minutes. Although these methods are simple and rapid, they are usually characterized by low sensitivity, and many control clinical samples show nonspecific agglutination.

  7. Agglutination is the clumping of particles. The word agglutination comes from the Latin agglutinare (glueing to). Agglutination is a reaction in which particles (as red blood cells or bacteria) suspended in a liquid collect into clumps usually as a response to a specific antibody.

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