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Fluorescence occurs when a photon of the incoming radiation is absorbed by an orbital electron in a molecule of the material, exciting it to a higher energy level. The electron then returns to its former energy level by losing energy, emitting another photon of a different frequency, which is the fluorescent glow.
Jan 30, 2023 · Fluorescence, a type of luminescence, occurs in gas, liquid or solid chemical systems. Fluorescence is brought about by absorption of photons in the singlet ground state promoted to a singlet excited state. The spin of the electron is still paired with the ground state electron, unlike phosphorescence.
Oct 9, 2024 · Fluorescence, emission of electromagnetic radiation, usually visible light, caused by excitation of atoms in a material, which then reemit almost immediately (within about 10−8 seconds).
- The Editors of Encyclopaedia Britannica
Apr 5, 2023 · Fluorescence is a phenomenon where certain materials rapidly (around 10-8 seconds) emit light when they are exposed to specific types of electromagnetic radiation, typically ultraviolet (UV) light. Fluorescent materials are those that can exhibit this characteristic.
Jun 2, 2023 · This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.
Nov 15, 2022 · By definition, fluorescence is a type of photoluminescence, which is what happens when a molecule is excited by ultraviolet or visible light photons. More specifically, fluorescence is the result of a molecule absorbing light at a specific wavelength and emitting light at a longer wavelength.
Fluorescence is the result of a three-stage process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes (Figure 1). A fluorescent probe is a fluorophore designed to respond to a specific stimulus or to localize within a specific region of a biological specimen.