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1. • • Q-Rt-PCR provides reliable measurements of BCR/abl fusion transcripts. It is potentially useful in assessing molecular residual disease after therapy.
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     Quantitative measurement of BCR/abl transcripts usingreal ...

2. People also ask

   Can quantitative real-time PCR detect bcr/abl fusion transcripts?

   • It provides not only detection but also quantification of the accumulated target PCR products. To validate quantitative real-time PCR (Q-Rt-PCR) in the detection of BCR/abl fusion transcripts, we performed side-by-side comparison analysis with conventional reverse transcription-mediated PCR (RT–PCR).

   Quantitative measurement of BCR/abl transcripts usingreal-time

   www.annalsofoncology.org/article/S0923-7534(19)62895-4/fulltext

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   Can BCR-ABL1 fusion transcripts be monitored by RT-PCR?

   • 2014 Mar;16 (2):174-9. doi: 10.1016/j.jmoldx.2013.10.007. Monitoring BCR-ABL1 fusion transcripts by real-time quantitative RT-PCR has become an important clinical test for the management of patients with chronic myeloid leukemia. However, it has some inherent limitations with regard to its lower limit of detection and limit of quantification.

   Detection and quantification of BCR-ABL1 fusion transcripts by ... - Pub…

   pubmed.ncbi.nlm.nih.gov/24389534/

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   Is Q-RT-PCR useful in detecting bcr/abl fusion transcripts in chronic myelogenous Leuk?

   • Lee 1 ∙ H. Kantarjian 2 ∙ A. Glassman 3 ∙ M. Talpaz 4 ∙ M.-S. Lee 1 Quantitative real-time polymerase chain reaction (Q-Rt-PCR) is a new tool in the detection and quantification of the BCR/abl fusion transcripts in chronic myelogenous leukemia (CML). This study investigates its specificity, sensitivity and potential clinical usefulness.

   Quantitative measurement of BCR/abl transcripts usingreal-time

   www.annalsofoncology.org/article/S0923-7534(19)62895-4/fulltext

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   How are BCR/ABL fusion transcripts normalized?

   • Consequently, the normalized measurements of the BCR/abl fusion transcripts in thetested samples were determined according to the following formula: BCR/abl norm = BCR/abl obsv × 1/RARα obsv. The rationale for the need of such normalization was that PCR efficiencies varied from sample to sample.

   Quantitative measurement of BCR/abl transcripts usingreal-time

   www.annalsofoncology.org/article/S0923-7534(19)62895-4/fulltext

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   Can a next-generation sequencing based assay quantify BCR ABL1 and ABL1 transcript copy numbers?

   • To overcome the limitations of the current standard real-time quantitative polymerase chain reaction (RQ-PCR), we designed and validated a next-generation sequencing (NGS)-based assay to quantify BCR::ABL1 and ABL1 transcript copy numbers.

   Development and validation of sensitive BCR::ABL1 fusion gene quantita…

   cancerci.biomedcentral.com/articles/10.1186/s12935-023-02938-2

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   Will Droplet digital RT-PCR be the preferred method for quantification of BCR-ABL1 fusion transcripts?

   • We expect the advantages of droplet digital RT-PCR will make it the preferred method for quantification of BCR-ABL1 fusion transcripts. Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

   Detection and quantification of BCR-ABL1 fusion transcripts by ... - Pub…

   pubmed.ncbi.nlm.nih.gov/24389534/

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3. www.nature.com › articles › s41375/022/01612-2Impact of BCR::ABL1 transcript type on RT-qPCR amplification ...

   www.nature.com › articles › s41375/022/01612-2

   Jun 8, 2022 · Branford S, Hughes TP, Rudzki Z. Monitoring chronic myeloid leukaemia therapy by real-time quantitative PCR in blood is a reliable alternative to bone marrow cytogenetics.

   • Author: Matthew Salmon

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5. pubmed.ncbi.nlm.nih.gov › 24389534Detection and quantification of BCR-ABL1 fusion transcripts ...

   pubmed.ncbi.nlm.nih.gov › 24389534

   Monitoring BCR-ABL1 fusion transcripts by real-time quantitative RT-PCR has become an important clinical test for the management of patients with chronic myeloid leukemia. However, it has some inherent limitations with regard to its lower limit of detection and limit of quantification.

   • Author: Lawrence J. Jennings, Lawrence J. Jennings, David George, Juliann Czech, Min Yu, Loren Joseph

   • Publish Year: 2014

6. pmc.ncbi.nlm.nih.gov › articles › PMC7115836Comparison of BCR-ABL1 quantification in peripheral blood and ...

   pmc.ncbi.nlm.nih.gov › articles › PMC7115836

   The reciprocal translocation t(9;22)(q34;q11) is associated with the BCR-ABL1 fusion that can be detected by fluorescence in situ hybridization (FISH) or reverse transcriptase polymerase chain reaction (RT-PCR) to establish the diagnosis .

7. cancerci.biomedcentral.com › articles › 10Development and validation of sensitive BCR::ABL1 fusion gene ...

   cancerci.biomedcentral.com › articles › 10

   • Cached

   May 29, 2023 · Real-time quantitative PCR (RQ-PCR) and droplet digital PCR (ddPCR) are used to quantify known fusion transcript types. RQ-PCR and ddPCR can detect major BCR::ABL1 transcripts. However, they did not differentiate between e13a2 and e14a2 types.

8. journals.plos.org › plosone › articleA new highly sensitive real-time quantitative-PCR method for ...

   journals.plos.org › plosone › article

   • Cached

   Mar 5, 2019 · In this study, we evaluated the ability of this in-house RQ-PCR method to detect low level BCR-ABL1 fusion transcripts using samples obtained in the ongoing Delightedly Overcome CML Expert Stop TKI (DOMEST) clinical trial to evaluate the rationale for cessation of imatinib .

9. www.jmdjournal.org › article › S1525-1578(13)00252-3Detection and Quantification of BCR-ABL1 Fusion Transcripts ...

   www.jmdjournal.org › article › S1525-1578(13)00252-3

   Several studies have demonstrated the clinical utility of quantifying BCR-ABL1 fusion transcript levels in chronic myeloid leukemia (CML) patients. 1–7 Typically, this has been performed using real-time quantitative RT-PCR (RT-qPCR).