CRISPR-Cas9 is a genome editing technology used to change parts of the genome. Learn how CRISPR-Cas9 works and discover its advantages and limitations.
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- In the context of cancer heterogeneity, the synergistic action of next-generation sequencing (NGS) and CRISPR/Cas9 plays a promising role in the personalized treatment of cancer. NGS enables high-throughput genomic profiling of tumors and pinpoints specific mutations that primarily lead to cancer.
pubmed.ncbi.nlm.nih.gov/38708901/Revolutionizing personalized cancer treatment: the synergy of ...
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Mar 24, 2022 · CRISPR/Cas9 genetic editing technology has been successfully employed for gene knock-in, gene knock-out, gene repair, and transcriptional regulation . In this review, we have discussed the potential role of CRISPR/Cas9 and NGS in the advancement of personalized cancer medicine.
- Comprehensive review of CRISPR-based gene editing: mechanisms ...
CRISPR-Cas9 is a powerful gene-editing tool that relies on...
- Comprehensive review of CRISPR-based gene editing: mechanisms ...
In the context of cancer heterogeneity, the synergistic action of next-generation sequencing (NGS) and CRISPR/Cas9 plays a promising role in the personalized treatment of cancer. NGS enables high-throughput genomic profiling of tumors and pinpoints specific mutations that primarily lead to cancer.
Jan 16, 2023 · Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases by...
- Tianxiang Li
- The evolution of CRISPR tools that have been harnessed in the investigation of cancer biology. Since the inception of CRISPR-associated 9 (Cas9) gene editing in mammalian cells, there has been a rapid expansion in the field of CRISPR technology.
- Different workflows used in CRISPR screening and mutagenesis. The CRISPR screening procedures commence by selecting the appropriate screening system, offering various options: A CRISPRko, where Cas9 is employed to disrupt genes, resulting in the generation of premature stop codons or frameshift mutations; CRISPRa, involving the attachment of activation domains (e.g., VPR, VP64) to dCas9, resulting in enhanced transcription of target genes; CRISPRi, on the contrary, employs repression domains (e.g., KRAB) tethered to dCas9, leading to a reduction in the transcription of target genes; Base editing screen, which uses a base editor (e.g., cytosine deaminase or adenine deaminase) with or without a uracil DNA glycosylase inhibitor to induce mutations without causing double-strand breaks.
- The various mechanisms employed for gene editing. In the first part (a), Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR-Cas systems are capable of generating double-strand breaks (DSBs) at precise locations within the genome.
- The diverse applications of CRISPR technology within cancer research. In section a, the paragraph explains that the inhibition of a specific gene can be accomplished by combining Deactivated Cas9 (dCas9) with repressor domains.
Mar 24, 2022 · Hence, in this review, we have discussed the potential role of CRISPR/Cas9 and NGS in advancing personalized medicine. With NGS and CRISPR/Cas9, the goal is no longer to match the treatment for the diagnosed tumor but rather to build a treatment method that fits the tumor exactly.
Feb 22, 2022 · This paper describes the development of a ‘PAMless’ CRISPR–Cas9 variant, SpRY, that dramatically broadens the number of possible genomic targets for nuclease- or non-nuclease-mediated editing.
Combining the CRISPR/Cas9 system with next-generation sequenc-ing (NGS) has the potential to speed up the treatment for cancer [3]. Gene editing techniques with CRISPR/Cas9 are based on creating double-strand breaks (DSBs) in specific genome sections, then repaired by cellular mechanisms.
