Physicians diagnose, treat, and prescribe medications via the phone or video consultation. See a doctor online and get scripts (re)filled at your nearest pharmacy, in 15 minutes.
Easy and convenient access to world-class healthcare - Yahoo Finance
Search results
Remote ischemic conditioning
- Remote ischemic conditioning (RIC) is an emerging and promising approach to attenuate postoperative renal IRI. In this strategy, several cycles of intermittent nonlethal ischemia and reperfusion are applied to a remote organ or issue, which could render the kidney more resistant against subsequent lethal ischemia and reperfusion [ 8 – 10 ].
journals.plos.org/plosone/article?id=10.1371/journal.pone.0170729Limited Clinical Utility of Remote Ischemic Conditioning in ...
May 3, 2024 · Remote ischemic conditioning (RIC) has the potential to benefit graft function following kidney transplantation by reducing ischemia-reperfusion injury; however, the current clinical evidence is inconclusive.
- Subclinical effects of remote ischaemic conditioning in human ...
Remote ischaemic conditioning (RIC) is currently being...
- Subclinical effects of remote ischaemic conditioning in human ...
- Context Patient Cohort and Ric Protocol
- Sample Preparation and Analysis by Mass Spectrometry
- Tissue Proteomics
- Plasma Proteomics
- Analysis by Mass Spectrometry
- Elisa Assays For SAA1 and CRP
- Measurements of S-Troponin and S-Myoglobin
- Proteomics Data Analysis
The CONTEXT trial concerned 225 included transplant recipients and evaluated effects of RIC, induced by episodes of obstruction to arterial flow in the leg on the opposite side of where the transplant was implanted using a tourniquet (4 × 5 min) on the thigh prior to kidney reperfusion . Trial registration: NCT01395719. Registered 14 July 2011, htt...
For this in-depth study to detect any underlying molecular changes due to RIC our identifier cohorts consisted of six pairs of recipients (Table 1). We have analysed tissue and plasma samples from these six kidney pairs (6 RIC and 6 control), at 2 and 4 time points, respectively. Pairs of kidneys from the same donor where the recipients were as sim...
Approximately 10 mg of kidney tissue (n = 6 per group, Table 1) was lysed in RIPA buffer (Pierce) using a bead beater homogenizer (Precellys). A BCA assay was performed and 50 µg of total protein from each sample was taken for Filter Aided Sample Preparation (FASP) digestion. In short, 30 kDa filters (Millipore) were equilibrated with 8 M urea buff...
One hundred microliter of plasma were precipitated by adding PEG 6000 to final concentration of 12% in order to deplete abundant plasma proteins. Depleted plasma protein concentrations (n = 6 per group, Table 1) were determined using a BCA assay (Pierce) and digested using a Thermo Scientific™ SMART Digest™ Kit. Briefly, 50 µg of protein was added ...
Liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis was performed using a Dionex Ultimate 3000 nano-ultra high pressure reversed-phase chromatography system coupled on-line to a Q Exactive High Field (HF) mass spectrometer (Thermo Scientific) as described previously . In brief, samples were separated on an EASY-Spray PepMap RSLC C18 ...
To validate proteomic findings, ELISA was used to detect and quantify SAA1 (R&D Systems, SAA1 DuoSet) levels in plasma of patients from the exploratory as well as the validation cohort where samples from all time points were available (n = 143, Table 1). Plates were coated overnight with capture antibody at RT and blocked with Reagent Diluent for 1...
In plasma from the whole CONTEXT cohort , ELISA assays were used to quantify levels of S-troponin T (Roche, Troponin T-high sensitive) and S-myoglobin (ADVIA Centaur XPT Immunoassay System) in plasma at baseline (TD0), 90 min and post-operative day 1 (TD1) according to the manufacturer’s instructions. As data are mixed with some paired data (when k...
MS raw data was searched using MaxQuant software (v1.3.1). The search parameters used were as follows: trypsin with two missed cleavages allowed, oxidation (M) and deamidation (N, Q) were set as variable modifications and carbamidomethylation (C) as a fixed modification. The data was searched against human protein sequences using the UPR_homoSapien...
- Adam M. Thorne, Honglei Huang, Darragh P. O‘Brien, Marco Eijken, Marco Eijken, Nicoline Valentina Kr...
- 2020
Remote ischaemic conditioning (RIC) improved early kidney graft function in a porcine model of donation after brain death and was associated with improved long-term cardiac outcome after myocardial ischaemia.
- Marie B Nielsen, Marie B Nielsen, Nicoline V Krogstrup, Nicoline V Krogstrup, Mihai Oltean, Gertrude...
- 10.1371/journal.pone.0226882
- 2019
- PLoS One. 2019; 14(12): e0226882.
Jul 9, 2024 · In our study, we investigated the role of RIC in the transplantation of multiple organs (heart, kidney, liver, and lung) and found that RIC, as an emerging therapeutic strategy, did not improve clinical outcomes in organ transplant patients.
Jan 27, 2017 · In summary, the present meta-analysis suggested that RIC might exert renoprotective functions in human kidney transplantation. However, the clinical utility remained limited, and further well-designed RCTs with large sample size are needed to assess this clinical efficacy and safety of RIC in renal transplantation.
- Chang-Cheng Zhou, Yu-Zheng Ge, Wen-Tao Yao, Ran Wu, Hui Xin, Tian-Ze Lu, Ming-Hao Li, Kai-Wei Song, ...
- 2017
Apr 5, 2023 · Objective: Studies have shown that remote ischemic conditioning (RIC) can effectively attenuate ischemic-reperfusion injury in the heart and brain, but the effect on ischemic-reperfusion injury in patients with kidney transplantation or partial nephrectomy remains controversial.
Apr 5, 2023 · ObjectiveStudies have shown that remote ischemic conditioning (RIC) can effectively attenuate ischemic-reperfusion injury in the heart and brain, but the effect on ischemic-reperfusion injury in patients with kidney transplantation or partial nephrectomy remains controversial.
