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2. chem.libretexts.org › Bookshelves › Organic_Chemistry2.3F: Visualizing TLC Plates - Chemistry LibreTexts

   chem.libretexts.org › Bookshelves › Organic_Chemistry

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   While wearing gloves and holding the TLC plate with forceps in a fume hood, quickly dip the plate into and out of the chemical dip jar so that the stain covers the area where the solvent traveled on the plate (Figure 2.34b).

3. chemistry.mcmaster.ca › adronov › resourcesStains for Developing TLC Plates - McMaster University

   chemistry.mcmaster.ca › adronov › resources

   This method is referred to as staining the TLC plate, and experience will allow you to determine what functional groups will appear as what color upon visualization. Following is a listing of the most commonly employed stains, the kind of compounds for which they're usually employed, and a typical recipe. A. Note.

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4. www.mamapedia.com › article › paper-plates-stainedPaper Plates "Stained" My Solid Cherry Table, Help!

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   May 3, 2011 · It is due to moisture and/or the heat of the plate which then leaves moisture. That is a water ring, damage. Hard to get rid of.

5. www.bbc.co.uk › bitesize › guidesThin layer chromatography - Chemical analysis - Higher ... - BBC

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   Thin layer chromatography (TLC) is similar to paper chromatography but instead of paper, the stationary phase is a thin layer of an inert substance (eg silica) supported on a flat, unreactive...

6. chem.libretexts.org › Courses › SUNY_Oneonta2.3E: Step-by-Step Procedures for Thin Layer Chromatography

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   Aug 11, 2020 · Add a portion of a prepared solvent for chromatography (Figure 2.23c, 5 - 10mL for this type of TLC chamber). Close the lid (or place the watch glass) and tilt the chamber to wet the filter paper. Obtain a TLC plate, touching the plate only on the back or edges, but not on the white surface.

7. chem.libretexts.org › Bookshelves › Organic_Chemistry2.3: Thin Layer Chromatography (TLC) - Chemistry LibreTexts

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   Visualization methods can be either non-destructive (compound is unchanged after the process) or destructive (compound is converted into something new after the process. Viewing a TLC plate under ultraviolet light is non-destructive, while using a chemical stain is destructive.

8. People also ask

   How do you remove stains from a TLC plate?

   • While wearing gloves and holding the TLC plate with forceps in a fume hood, quickly dip the plate into and out of the chemical dip jar so that the stain covers the area where the solvent traveled on the plate (Figure 2.34b). Let excess liquid drip off the plate for a few seconds, then wipe the back of the plate with a paper towel (Figure 2.34c).

   2.3F: Visualizing TLC Plates - Chemistry LibreTexts

   chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_Lab_Techniques_(Nichols)/02:_Chromatography/2.03:_Thin_Layer_Chromatography_(TLC)/2.3F:_Visualizing_TLC_Plates

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   What stains are used to visualize a plate?

   • The plate is visualized with UV light (Figure 2.36b), p -anisaldehyde stain (Figure 2.36c), and vanillin stain (Figure 2.36d). 4-heptanone (lane #1) and acetophenone (lane #2) showed similar colorations using the two stains.

   2.3F: Visualizing TLC Plates - Chemistry LibreTexts

   chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_Lab_Techniques_(Nichols)/02:_Chromatography/2.03:_Thin_Layer_Chromatography_(TLC)/2.3F:_Visualizing_TLC_Plates

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   Does staining a plate support an acidic spot?

   • Staining of the plate with bromocresol green (a stain for acidic compounds), supports this hypothesis as the lower acidic spot is visualized with this method (Figure 2.48b). This is an example of when the staining results "make structural sense", and can even support the identification of unknown spots.

   2.3F: Visualizing TLC Plates - Chemistry LibreTexts

   chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_Lab_Techniques_(Nichols)/02:_Chromatography/2.03:_Thin_Layer_Chromatography_(TLC)/2.3F:_Visualizing_TLC_Plates

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   How do you identify spots on a plate?

   • Vigorous heating is required to develop the spots, but the plate is overheated when the background begins to darken. There is typically no color differentiation between spots, as most compounds visualize as green or blue spots (Figure 2.41c).

   2.3F: Visualizing TLC Plates - Chemistry LibreTexts

   chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_Lab_Techniques_(Nichols)/02:_Chromatography/2.03:_Thin_Layer_Chromatography_(TLC)/2.3F:_Visualizing_TLC_Plates

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   What stains can be visualized using a TLC plate?

   • Alcohols, amines, sulfides, mercaptans and other oxidizable functional groups may also be visualized, however it will be necessary to gently heat the TLC plate following immersion into the stain. These spots will appear as either yellow or light brown on a light purple or pink background.

   Stains for Developing TLC Plates | McMaster University

   chemistry.mcmaster.ca/adronov/resources/Stains_for_Developing_TLC_Plates.pdf

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   How do you store a blue stain?

   • This stain is light sensitive and should be stored wrapped in aluminum foil in the refrigerator. It is originally light yellow, but darkens over time (Figure 2.37f+g). It should be discarded if it acquires a blue color. Safety note: wear gloves while using this highly acidic stain.

   2.3F: Visualizing TLC Plates - Chemistry LibreTexts

   chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_Lab_Techniques_(Nichols)/02:_Chromatography/2.03:_Thin_Layer_Chromatography_(TLC)/2.3F:_Visualizing_TLC_Plates

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9. www.abbottsathome.com › remove-scratches-on-platesTesting 6 Ways To Remove Scratches On Plates And Dishes: One ...

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   Next up is Hydrogen Peroxide. It’s great for cleaning grout and even oil stains on fabric. So, I thought it might work on silverware scratches on plates too. For this test I put a paper towel over the plate, then soaked the paper towel in Hydrogen Peroxide. The paper towel keeps the whole plate wet, instead of all of the liquid pooling in the ...