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1. www.nature.com › articles › nprotProcessing tissue and cells for transmission electron ...

   www.nature.com › articles › nprot

   Oct 4, 2007 · TEM also has an important role in cytopathology. Cells in effusions, like cells in culture, can be readily prepared for TEM. Epithelial cells are easily distinguishable from mesothelial...

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2. www.nature.com › articles › s41598/020/79637-9TEM, SEM, and STEM-based immuno-CLEM workflows offer ... - Nature

   www.nature.com › articles › s41598/020/79637-9

   Jan 13, 2021 · Combining our iCLEM-TEM and TEM immuno-gold workflows provides a powerful method to conclusively identify tissue stem cells and characterize their ultrastructural features.

   • Author: Viola Oorschot, Benjamin W Lindsey, Benjamin W Lindsey, Jan Kaslin, Georg Ramm

   • Publish Year: 2021

3. acsjournals.onlinelibrary.wiley.com › doi › fullCurrent role of cytopathology in the molecular and ...

   acsjournals.onlinelibrary.wiley.com › doi › full

   May 15, 2024 · Cytopathology has evolved significantly in the molecular and computational era, integrating next-generation sequencing techniques and artificial intelligence tools to enhance diagnostic accuracy and ...

   • Cited By: 1

   • Published: 15 May 2024

4. pubmed.ncbi.nlm.nih.gov › 17947985Processing tissue and cells for transmission electron ...

   pubmed.ncbi.nlm.nih.gov › 17947985

   In transmission electron microscopy (TEM), electrons are transmitted through a plastic-embedded specimen, and an image is formed. TEM enables the resolution and visualization of detail not apparent via light microscopy, even when combined with immunohistochemical analysis.

   • Author: Lesley Graham, Jan Marc Orenstein

   • Publish Year: 2007

5. pubmed.ncbi.nlm.nih.gov › 33441723TEM, SEM, and STEM-based immuno-CLEM workflows offer ...

   pubmed.ncbi.nlm.nih.gov › 33441723

   Jan 13, 2021 · Taking advantage of the antigenicity preservation of the Tokuyasu technique, we have established robust protocols and workflows and provide a side-by-side comparison of iCLEM used in combination with scanning EM (SEM), scanning TEM (STEM), or transmission EM (TEM).

   • Author: Viola Oorschot, Benjamin W Lindsey, Benjamin W Lindsey, Jan Kaslin, Georg Ramm

   • Publish Year: 2021

6. www.nature.com › articles › s41563/020/00833-zTowards data-driven next-generation transmission electron ...

   www.nature.com › articles › s41563/020/00833-z

   Oct 26, 2020 · TEM provides rich, directly resolved information about the structure and dynamics of phenomena spanning atoms to micrometres that are of great fundamental and practical significance to society.

7. People also ask

   How does TEM work?

   • Details of fixation, embedding and how to prepare thin and semi-thin sections, which can be used for analysis complementary to that performed ultimately using TEM, are also described. In transmission electron microscopy (TEM), electrons are transmitted through a plastic-embedded specimen, and an image is formed.

   Processing tissue and cells for transmission electron microscopy in diag…

   pubmed.ncbi.nlm.nih.gov/17947985/

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   Why is TEM important?

   • TEM continues to play a critical role in discovering and describing new organisms, especially viruses, and rendering viral diagnosis in, for example, diarrheal diseases. It has provided an understanding of the structure and organization of the components of tissue, cells and organisms.

   Processing tissue and cells for transmission electron microscopy in

   www.nature.com/articles/nprot.2007.304

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   How do cytochemical cell markers and ultrastructural morphology help identify stem cell populations?

   • To distinguish and molecularly characterise stem cell populations in large heterogeneous tissues, the combination of cytochemical cell markers with ultrastructural morphology is highly beneficial. Here, we realise this through workflows of multi-resolution immuno -correlative light and electron microscopy (i CLEM) methodologies.

   TEM, SEM, and STEM-based immuno-CLEM workflows offer ... - Nature

   www.nature.com/articles/s41598-020-79637-9

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   What is transmission electron microscopy (TEM)?

   • Provided by the Springer Nature SharedIt content-sharing initiative In transmission electron microscopy (TEM), electrons are transmitted through a plastic-embedded specimen, and an image is formed. TEM enables the resolution and visualization of detail not apparent via light microscopy, even when combined with immunohistochemical analysis.

   Processing tissue and cells for transmission electron microscopy in

   www.nature.com/articles/nprot.2007.304

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   What is a TEM test used for?

   • Ultrastructural examination of tissues, cells and microorganisms plays a vital role in diagnostic pathology and biologic research. TEM is used to study the morphology of cells and their organelles, and in the identification and characterization of viruses, bacteria, protozoa and fungi.

   Processing tissue and cells for transmission electron microscopy in

   www.nature.com/articles/nprot.2007.304

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   What is a TEM method?

   • In this protocol, we present a TEM method for preparing specimens obtained in clinical or research settings, discussing the particular requirements for tissue and cell preparation and analysis, the need for rapid fixation and the possibility of analysis of tissue already fixed in formalin or processed into paraffin blocks.

   Processing tissue and cells for transmission electron microscopy in

   www.nature.com/articles/nprot.2007.304

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8. www.sciencedirect.com › topics › neuroscienceTransmission Electron Microscopy - an ... - ScienceDirect

   www.sciencedirect.com › topics › neuroscience

   Transmission Electron Microscopy (TEM) is a technique that uses an electron beam to pass through a sample, providing high-resolution images of its size and structure. It is commonly used for imaging exosomes and has contributed to the understanding of biomolecules in their natural state.