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- TEM enables the resolution and visualization of detail not apparent via light microscopy, even when combined with immunohistochemical analysis. Ultrastructural examination of tissues, cells and microorganisms plays a vital role in diagnostic pathology and biologic research.
www.nature.com/articles/nprot.2007.304
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Oct 4, 2007 · TEM also has an important role in cytopathology. Cells in effusions, like cells in culture, can be readily prepared for TEM. Epithelial cells are easily distinguishable from mesothelial...
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INTRODUCTION. Transmission electron microscopy (TEM) requires extensive preparation of plant tissues to achieve optimal results. Samples must be fixed to preserve ultrastructure, dehydrated to withstand the high vacuum of an electron microscope, and embedded in a suitable resin to permit very thin sections to be produced.
Jan 13, 2021 · Combining our iCLEM-TEM and TEM immuno-gold workflows provides a powerful method to conclusively identify tissue stem cells and characterize their ultrastructural features.
- Viola Oorschot, Benjamin W Lindsey, Benjamin W Lindsey, Jan Kaslin, Georg Ramm
- 2021
Two types of electron microscope have been used to study plant cells in culture, the transmission (TEM) and scanning (SEM) electron microscopes. With the TEM, the electron beam penetrates thin slices of biological material and permits the study of internal features of cells and organelles.
- Larry C. Fowke
- 1995
Transmission electron microscopy (TEM) is an important tool for observing the ultrastructure of plant virions and their host cells. The two main applicable TEM technologies used in plant virology are negative staining and ultrathin section.
Aug 18, 2018 · Moreover, nowadays, we have new branches of transmission electron microscopy that enhance preparation and imaging of the samples; e.g. TEM tomography can generate semi-3D reconstructions (±60/70°) of plant cells and tissues at nanometer resolutions, while cryo-TEM allows visualization of frozen unstained samples (no reagents added during the ...
Nov 17, 2018 · Electron tomography (ET) approaches are based on the imaging of a biological specimen at different tilt angles by transmission electron microscopy (TEM). ET can be applied to both plastic-embedded and frozen samples.
