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Electrons are brought to focus on a phosphorescent screen (which has crystals that emit visible light). Image formation depends on differential scattering of electrons within the specimen, an effect that is proportional to the sizes of atomic nuclei in the specimen (Figure 7.1). Biological material mainly consists of atoms of low atomic number.
The TEM has revealed structures in cells that are not visible with the light microscope. SEMs are often used at lower magnifications (up to ×30,000). The limit of resolution of a SEM is lower...
Oct 4, 2007 · In transmission electron microscopy (TEM), electrons are transmitted through a plastic-embedded specimen, and an image is formed. TEM enables the resolution and visualization of detail not...
- Lesley Graham, Jan Marc Orenstein
- 2007
The basic subcellular structures of an eukaryotic cell as seen by transmission electron microscopy (TEM). Try to identify the cell borders and nuclei. Switch between the grayscale and color images (see above) to help identify the following structures: Plasma Membranes (dark green) - portions visible in cells 1, 2 and 4
Transmission electron microscope image of a human leukocyte (also known as a white blood cell), showing the Golgi apparatus, which is a structure involved in protein transport in the cytoplasm of the cell.
Figure 01-08: TEM micrograph showing a 70,000× magnified portion of the cytoplasm of a plant cell. The ER, a Golgi stack, and some vesicles are visible. The structures are near the edge of the cell, as a cell wall is visible on the right-hand side of the image.
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In the cytoplasm, to the right of the nucleus, are several mitochondria which have been sectioned in different planes; the internal membranes of the organelle appear dark against the paler matrix. Also present in the cytoplasm are the fluid-filled vesicles and flattened sacs of the Golgi apparatus.
