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The TEM has revealed structures in cells that are not visible with the light microscope. SEMs are often used at lower magnifications (up to ×30,000). The limit of resolution of a SEM is lower...
The basic subcellular structures of an eukaryotic cell as seen by transmission electron microscopy (TEM). Try to identify the cell borders and nuclei. Switch between the grayscale and color images (see above) to help identify the following structures: Plasma Membranes (dark green) - portions visible in cells 1, 2 and 4
Figure 01-01: Examples of the four different types of microscopy, imaging green algae cells (species unknown): brightfield light microscopy, fluorescence light microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). An average algal cell is between 2 and 7 µm.
Three-dimensional views of the surfaces of cells and tissues are obtained by scanning electron microscopy. The shapes of isolated macromolecules that have been shadowed with a heavy metal or outlined by negative staining can also be readily determined by electron microscopy.
- Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter
- 2002
- 2002
Transmission electron microscopy (TEM) produces two-dimensional images of a specimen by imaging a thin section with a beam of electrons. Ultrathin tissue sections are stained with heavy metals (such as osmium tetroxide, uranium or lead salts) to enhance contrast.
Image formation depends on differential scattering of electrons within the specimen, an effect that is proportional to the sizes of atomic nuclei in the specimen (Figure 7.1). Biological material mainly consists of atoms of low atomic number.
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The transmission electron microscope (TEM) is used to examine thin slices or sections of cells or. tissues. . TEMs have a maximum magnification of around x1,000,000, but images can be enlarged...
