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- The nucleus is at the top and the cytoplasm at the bottom. The inner and the outer membrane of the nuclear envelope may be seen as two parallel lines in the middle of the image.
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The TEM has revealed structures in cells that are not visible with the light microscope. SEMs are often used at lower magnifications (up to ×30,000). The limit of resolution of a SEM is lower...
Figure 01-01: Examples of the four different types of microscopy, imaging green algae cells (species unknown): brightfield light microscopy, fluorescence light microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). An average algal cell is between 2 and 7 µm.
The basic subcellular structures of an eukaryotic cell as seen by transmission electron microscopy (TEM). Try to identify the cell borders and nuclei. Switch between the grayscale and color images (see above) to help identify the following structures: Plasma Membranes.
Three-dimensional views of the surfaces of cells and tissues are obtained by scanning electron microscopy. The shapes of isolated macromolecules that have been shadowed with a heavy metal or outlined by negative staining can also be readily determined by electron microscopy.
- Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter
- 2002
- 2002
May 31, 2022 · Notice the lack of a cell wall. TEM electron micrograph of a plant cell showing key features. Notice the presence of a cell wall and vacuole. Mucus producing goblet cells (found in the lining of trachea, bronchi and larger bronchioles) are shown in a photomicrograph.
Image formation depends on differential scattering of electrons within the specimen, an effect that is proportional to the sizes of atomic nuclei in the specimen (Figure 7.1). Biological material mainly consists of atoms of low atomic number.
The TEM has revealed structures in cells that are not visible with the light microscope. Image caption, A human lymphocyte white blood cell as seen with a transmission electron microscope
