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Feb 18, 2021 · Astrocytic processes have a clear, electron lucent cytoplasm, while oligodendrocytic and microglial processes have a moderately dense cytoplasm, and neurons have a cytoplasm showing intermediate gray levels between astrocytes and the other two glial cells (Peters et al., 1990).
Oct 6, 2021 · Electron microscopy (EM) uniquely visualizes cellular structures with nanometre resolution. However, traditional methods, such as thin-section EM or EM tomography, have limitations in that they...
- C. Shan Xu, Song Pang, Gleb Shtengel, Andreas Müller, Alex T. Ritter, Huxley K. Hoffman, Huxley K. H...
- 2021
Once dried, specimens are coated with a thin layer of gold or gold-palladium and placed in the microscope. Figure 7.3 Example of a SEM image. A cast of the blood vessels in a rat brain was made using resins purchased from VasQtec, gold sputtered and imaged inside a scanning electron microscope.
In the case of fibrous astrocytes, which are more abundant in layer I and in the deep layers of the cortex close to the white matter, bundles of fibrils can be visible in the cytoplasm and may correspond to the pinkish threads that are sometimes visible in Nissl stained tissue.
Transmission electron microscopy (TEM) images from ~30–300 nm diamond-knife cut sections of metal stained samples provide convenient high-resolution visualization on a small fraction of the whole cell, which have profoundly shaped our understanding of organelles 4. Series of sections can be digitally stitched together to form a 3D volume, but the 30 nm or larger sampling interval sacrifices ...
Mar 2, 2020 · Preservation of the nuclear envelope (arrows) was improved by sample processing with high contrast en-bloc stain, which resulted in intact and essentially parallel double membranes with clearly visible nuclear pores (arrowhead). N: nucleus, C: Cytoplasm. Scale bars: 100 nm.
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Oct 4, 2007 · TEM is also used to visualize chromatin and DNA in its various forms, while freeze-fracture techniques have been used to image cell surfaces and also the contents of cellular organelles.
