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Feb 29, 2012 · Cells viewed with the TEM. Transmission electron microscope image of a human leukocyte (also known as a white blood cell), showing the Golgi apparatus, which is a structure involved in protein transport in the cytoplasm of the cell.
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Joseph Jackson Lister reduces spherical aberration (which...
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The basic subcellular structures of an eukaryotic cell as seen by transmission electron microscopy (TEM). Try to identify the cell borders and nuclei. Switch between the grayscale and color images (see above) to help identify the following structures: Plasma Membranes (dark green) - portions visible in cells 1, 2 and 4
Figure 01-01: Examples of the four different types of microscopy, imaging green algae cells (species unknown): brightfield light microscopy, fluorescence light microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). An average algal cell is between 2 and 7 µm.
In Transmission Electron Microscopy (TEM), we detect electrons that have interacted with atoms in the sample as they passed through it, producing a “projection” image of the 3D object onto a 2D plane, similar to a medical X-ray image. This shows details throughout the cell, not just on the surface.
Transmission Electron Microscopy. Ultrathin section of a fibroblast as seen by transmission electron microscope (TEM). Examine the ultrastructure of this spindle-shaped cell: Plasma Membrane; Nucleus. Nucleolus is not visible; Golgi Apparatus. Stacks of membranes surrounded by vesicles; Mitochondria. Many cisterna are seen within the mitochondria
In the cytoplasm, to the right of the nucleus, are several mitochondria which have been sectioned in different planes; the internal membranes of the organelle appear dark against the paler matrix. Also present in the cytoplasm are the fluid-filled vesicles and flattened sacs of the Golgi apparatus.
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The TEM has revealed structures in cells that are not visible with the light microscope. SEMs are often used at lower magnifications (up to ×30,000). The limit of resolution of a SEM is lower...