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The basic subcellular structures of an eukaryotic cell as seen by transmission electron microscopy (TEM). Try to identify the cell borders and nuclei. Switch between the grayscale and color images (see above) to help identify the following structures: Plasma Membranes (dark green) - portions visible in cells 1, 2 and 4
the transmission electron microscope (TEM) is used to examine thin slices or sections of cells or. tissues. the scanning electron microscope (SEM) has a large. depth of field. so can be used...
Figure 01-08: TEM micrograph showing a 70,000× magnified portion of the cytoplasm of a plant cell. The ER, a Golgi stack, and some vesicles are visible. The structures are near the edge of the cell, as a cell wall is visible on the right-hand side of the image.
Electron Microscopy (TEM) or can look at the outer surface of a sample using Scanning Electron Microscopy (SEM), analogous to a stereo light microscope. 7.1.2 Transmission electron microscopy (TEM) The greater resolving power of electron microscopes derives from the wave properties of electrons.
There is little in the contents of most cells (which are 70% water by weight) to impede the passage of light rays. Thus, most cells in their natural state, even if fixed and sectioned, are almost invisible in an ordinary light microscope. One way to make them visible is to stain them with dyes.
- Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter
- 2002
- 2002
In Transmission Electron Microscopy (TEM), we detect electrons that have interacted with atoms in the sample as they passed through it, producing a “projection” image of the 3D object onto a 2D plane, similar to a medical X-ray image. This shows details throughout the cell, not just on the surface.
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In the cytoplasm, to the right of the nucleus, are several mitochondria which have been sectioned in different planes; the internal membranes of the organelle appear dark against the paler matrix. Also present in the cytoplasm are the fluid-filled vesicles and flattened sacs of the Golgi apparatus.
