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Figure 01-08: TEM micrograph showing a 70,000× magnified portion of the cytoplasm of a plant cell. The ER, a Golgi stack, and some vesicles are visible. The structures are near the edge of the cell, as a cell wall is visible on the right-hand side of the image.
The TEM has revealed structures in cells that are not visible with the light microscope. SEMs are often used at lower magnifications (up to ×30,000). The limit of resolution of a SEM is lower...
The basic subcellular structures of an eukaryotic cell as seen by transmission electron microscopy (TEM). Try to identify the cell borders and nuclei. Nuclear Envelope (purple) - composed of an inner and outer nuclear membrane. (green) - ribosomes appear as clusters of small, dark particles.
Examine the cytoplasm (green) for polyribosomes (dark particles) in spiral and linear patterns.
In overall design the transmission electron microscope (TEM) is similar to a light microscope, although it is much larger and upside down (Figure 9-22). The source of illumination is a filament or cathode that emits electrons at the top of a cylindrical column about 2 m high.
- Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter
- 2002
- 2002
In Transmission Electron Microscopy (TEM), we detect electrons that have interacted with atoms in the sample as they passed through it, producing a “projection” image of the 3D object onto a 2D plane, similar to a medical X-ray image. This shows details throughout the cell, not just on the surface.
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The TEM has revealed structures in cells that are not visible with the light microscope. Image caption, A human lymphocyte white blood cell as seen with a transmission electron microscope
