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Figure 01-08: TEM micrograph showing a 70,000× magnified portion of the cytoplasm of a plant cell. The ER, a Golgi stack, and some vesicles are visible. The structures are near the edge of the cell, as a cell wall is visible on the right-hand side of the image.
The basic subcellular structures of an eukaryotic cell as seen by transmission electron microscopy (TEM). Try to identify the cell borders and nuclei. Nuclear Envelope (purple) - composed of an inner and outer nuclear membrane. (green) - ribosomes appear as clusters of small, dark particles.
In overall design the transmission electron microscope (TEM) is similar to a light microscope, although it is much larger and upside down (Figure 9-22). The source of illumination is a filament or cathode that emits electrons at the top of a cylindrical column about 2 m high.
- Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter
- 2002
- 2002
Examine the cytoplasm (green) for polyribosomes (dark particles) in spiral and linear patterns.
In Transmission Electron Microscopy (TEM), we detect electrons that have interacted with atoms in the sample as they passed through it, producing a “projection” image of the 3D object onto a 2D plane, similar to a medical X-ray image. This shows details throughout the cell, not just on the surface.
Electron Microscopy (TEM) or can look at the outer surface of a sample using Scanning Electron Microscopy (SEM), analogous to a stereo light microscope. 7.1.2 Transmission electron microscopy (TEM) The greater resolving power of electron microscopes derives from the wave properties of electrons.
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Transmission electron microscopy (TEM) offers the possibility of visualizing biological structures at resolution well beyond that of light microscopy.
