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Mar 16, 2022 · Gram-positive bacteria have cell walls that contain thick layers of peptidoglycan, a substance that forms the cell walls of many bacteria. The peptidoglycan forms about 90% of the cell wall in gram-positive bacteria. This causes them to appear blue to purple under a Gram stain. Gram-positive organisms include: Staphylococcus species.
Aug 10, 2022 · Procedure of Gram Staining. Take a clean, grease free slide. Prepare the smear of suspension on the clean slide with a loopful of sample. Crystal Violet was poured and kept for about 30 seconds to 1 minutes and rinse with water. Flood the gram’s iodine for 1 minute and wash with water. Then ,wash with 95% alcohol or acetone for about 10-20 ...
Aug 14, 2023 · The Gram staining is one of the most crucial staining techniques in microbiology. It gets its name from the Danish bacteriologist Hans Christian Gram, who first introduced it in 1882, mainly to identify organisms causing pneumonia.[1] Often, the first test performed, gram staining, involves the use of crystal violet or methylene blue as the primary color.[2] The term for organisms that retain ...
- Nishant Tripathi, Amit Sapra
- 2023/08/14
Apr 21, 2024 · Figure \(\PageIndex{1}\): Gram-staining is a differential staining technique that uses a primary stain and a secondary counterstain to distinguish between gram-positive and gram-negative bacteria. The purple, crystal-violet stained cells are referred to as gram-positive cells, while the red, safranin-dyed cells are gram-negative (Figure \(\PageIndex{4}\)).
Feb 9, 2022 · The role of the Gram staining procedure is crucial in bacteriology. It is used to distinguish between Gram-positive and Gram-negative bacteria based on differential staining with a crystal violet-iodine complex (CV-I) and a safranin counterstain. The cell wall of gram-positive bacteria retain the CV-I complex after treatment with ethyl alcohol and appear purple, but gram-negative bacteria ...
Mar 14, 2024 · Gram Stain Procedure. Place a smear or drop of culture on a slide and allow to completely air dry. Heat-fix the slide by drawing it quickly over the flame of a Bunsen burner. Put a few drops of crystal violet on the fixed culture and allow to sit for one minute. Gently rinse with distilled water over a sink.
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Both Gram-positive and Gram-negative bacteria become directly stained and appear purple after this step. 2. The bacteria are then treated with Gram's iodine solution. This allows the stain to be retained better by forming an insoluble crystal violet-iodine complex. Both Gram-positive and Gram-negative bacteria remain purple after this step. 3.
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