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Feb 10, 2014 · Conserved DNA uptake. Gram-positive and Gram-negative bacteria rely on highly similar DNA-uptake systems, with the only major difference being the requirement for transport across the outer ...
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1 day ago · In Gram-positive Firmicutes, it also has a role in natural transformation, while in Gram-negative bacteria, ComM is the canonical transformation-specific helicase. Both RadA and ComM helicases form hexameric rings and use ATP hydrolysis as an energy source to propel themselves along DNA.
Dec 2, 2005 · In Gram-negative bacteria, dsDNA enters the periplasm, but in both Gram-negative and -positive systems, a single strand of DNA passes across the cytoplasmic membrane while its complement is degraded (Fig. 1A). DNA is taken up into the cytosolic space linearly , and a free end is presumably required to initiate the transport process.
- Inês Chen, Peter J. Christie, David Dubnau
- 10.1126/science.1114021
- 2005
- 2005/12/12
Jun 23, 2020 · 2. Resistance of Gram-Positive Bacteria. The resistance mechanism of Gram-positive bacteria can occur through two major strategies: enzymatic degradation of antibiotic by the production of β-lactamases, or by decreasing the affinity and susceptibility of their target site, the penicillin-binding protein (PBP), by either acquisition of exogenous DNA or by changes in the native PBP genes [9,10].
- Buthaina Jubeh, Zeinab Breijyeh, Rafik Karaman
- 10.3390/molecules25122888
- 2020
- Molecules. 2020 Jun; 25(12): 2888.
Aug 14, 2019 · Transformation in Gram-positive (a) and Gram-negative bacteria (b).(a) Gram-positive bacteria: (i) Induction of competence, competence factor (yellow triangle) is produced and either released into the culture medium (Streptococcus) or remains cell-bound (Bacillus); (ii) Nucleases and eclipse proteins are produced; (iii) Random dsDNA degradation to generate ssDNA that binds onto choline-rich ...
- T. G. Villa, L. Feijoo-Siota, A. Sánchez-Pérez, Jl. R. Rama, C. Sieiro
- 2019
Mar 1, 2004 · In Gram-negative bacteria, this protection can be achieved by crossing the outer membrane; by contrast, in Gram-positive bacteria, DNA uptake is synonymous with passage across the cytoplasmic ...
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Nov 3, 2021 · The outer membrane is thought to be essential for viability in most Gram-negative bacteria 1. Owing to its adjacency with the extracellular milieu, the outer membrane serves as the location of a ...
