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To assess the functional relevance of this interaction in the context of replication forks, we adopted a previously established approach based on the displacement of endogenous replication factors by their recombinant versions, which are preferentially incorporated into replicating DNA (Simon et al., 2016).
Jun 17, 2024 · We can measure DNA replication speeds in single cells by using a double EdU pulse in combination with a mixture model. Representative cells labeled with a double EdU pulse (Δ t = 75 min ...
Mar 1, 2002 · The Meselson and Stahl experiment is still in wide use today. It has been used to demonstrate the distributive nature of histone deposition during chromosome replication and most recently to...
- Bruce Stillman
- 2002
Jan 3, 2018 · Between 1953 and 1957, before the Meselson-Stahl experiment verified semi-conservative replication of DNA, scientists debated how DNA replicated. In 1953, James Watson and Francis Crick proposed that DNA was composed of two helical strands that wound together in a coil.
These technical breakthroughs in genetic engineering—the ability to manipulate DNA with precision in a test tube or an organism—have had a dramatic impact on all aspects of cell biology by facilitating the study of cells and their macromolecules in previously unimagined ways.
- Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter
- 2002
- 2002
DNA Replication Mechanisms. All organisms must duplicate their DNA with extraordinary accuracy before each cell division. In this section, we explore how an elaborate “replication machine” achieves this accuracy, while duplicating DNA at rates as high as 1000 nucleotides per second. Go to: Base-Pairing Underlies DNA Replication and DNA Repair.
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explain how DNA is replicated accurately. explain how and why damage to DNA is repaired. Living cells must be able to duplicate their entire set of genetic instructions every time they divide. Likewise, multicellular organisms must be able to pass on complete copies of their genetic information to future generations.
