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Apr 17, 2012 · Based on our analysis of a diverse collection of 16 S rRNA gene sequences, we designed a broad-coverage quantitative real-time PCR (qPCR) assay—BactQuant—for quantifying 16 S rRNA gene copy number and estimating bacterial load. We further utilized in silico evaluation to complement laboratory-based qPCR characterization to validate BactQuant.
- Cindy M. Liu, Cindy M. Liu, Maliha Aziz, Sergey Kachur, Sergey Kachur, Po Ren Hsueh, Yu Tsung Huang,...
- 2012
Apr 29, 2021 · A roadmap for next-generation functional microbiology. Identifying and then proving that a genetic variant causes a phenotypic change is a considerable step towards understanding bacterial genomics. However, determining the precise role of the gene and how the encoded protein may function requires further study.
- Carolin M Kobras, Andrew K Fenton, Samuel K Sheppard
- 2021
Apr 21, 2024 · Describe the discovery of nucleic acid and nucleotides. Explain the historical experiments that led to the characterization of DNA. Describe how microbiology and microorganisms have been used to discover the biochemistry of genes. Explain how scientists established the link between DNA and heredity.
Dec 11, 2006 · In the next 8 chapters, the author provides basic principles and specific details on plasmids and bacteriophages as vectors for gene cloning, DNA purification techniques, enzymatic manipulation of purified DNA, its introduction into living cells, cloning vectors for Escherichia coli and eukaryotes, analysis of gene clones, and, finally, PCR.
Contents. Preface. Nucleic Acid Structure and Function. 1.1 Structure of nucleic acids. 1.1.1 DNA. 1.1.2 RNA. 1.1.3 Hydrophobic interactions. 1.1.4 Different forms of the double helix. 1.1.5 Supercoiling.
Nov 9, 2021 · We produced a searchable COmpact Bit-sliced Signature (COBS) index, facilitating the easy interrogation of the entire dataset for a specific sequence (e.g., gene, mutation, or plasmid). Additional MinHash and pp-sketch indices support genome-wide comparisons and estimations of genomic distance.
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Nov 10, 2021 · In a new study, from the Wellcome Sanger Institute and EMBL's European Bioinformatics Institute (EMBL-EBI), researchers standardized all bacterial genome data held in the European Nucleotide...
