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- Cell Lines
- Chemicals and Other Reagents
- Flow Cytometry
- Western Blot
- Patients and Clinical Samples
- Immunohistochemical Staining of Patient Samples
- NanoString Assay of Patient Samples
- Statistical Analysis
We employed three colon cancer cell lines, SW480, SW620, and DLD-1 in our study. Both SW480 and SW620 were MSS, while the DLD-1 was MSI-H cell line . All cell lines were purchased from the American Type Culture Collection. The cells were planted in T75 culture flasks (Thermo Fisher Scientific, 156499, Waltham, MA, USA) and maintained in RPMI-1640 m...
The compounds applied in this study for colon cancer cell lines were bortezomib (Cayman Chemical, 10008822, Ann Arbor, MI, USA), N-acetyl-L-leucyl-L-leucyl-L-norleucinal (LLnL, Sigma-Aldrich, 208719, Darmstadt, Germany), MG132 (Sigma-Aldrich, 474790), and IFN-γ (R&D Systems, 285-IF-100, Minneapolis, MN, USA). The chemicals applied for this experime...
The antibodies applied for flow cytometry were phycoerythrin (PE) anti-human pan-MHC class I (BioLegend, 311406, San Diego, CA, USA), purified anti-human HLA-A (HLA-A; MyBioSource, MBS438658), PE anti-mouse immunoglobulin G1 (IgG1; BioLegend 406608), purified anti-human HLA-C (HLA-C; BioLegend, 373302), PE anti-mouse IgG2b (IgG2b; BioLegend, 406708...
We applied enhanced chemiluminescence Immobilon Crescendo Western horseradish peroxidase (HRP) substrate (Merck Millipore, WBLUR0500, Darmstadt, Germany) for these Western blots. The antibodies applied for Western blots were STAT1 (Cell Signaling Technology, #9172, Danvers, MA, USA), phosphorylated STAT1 (pSTAT1; Cell Signaling Technology, #9167), ...
We enrolled patients at National Taiwan University Hospital with the following eligibility criteria: (1) patients aged ≥ 20 years; (2) patients with metastatic CRC; and (3) patients without known history of receiving immune modification drugs, such as steroids, immunosuppressive agents, and immunotherapy under clinical trial settings. Informed cons...
The IHC staining procedures were detailed in the earlier study . Briefly summarized, we applied the Opal 7 Solid Tumor Immunology Kit (PerkinElmer, OP7TL4001KT) for multiplex IHC staining. The PerkinElmer Opal multiplex system could simultaneously apply up to 7 different IHC staining for each cell. The 4′,6-diamidino-2-phenylindole (DAPI) was appli...
We applied a commercial multiplexed gene expression panel, the NanoString nCounter PanCancer Immune Profiling Panel (NanoString Technologies, Seattle, WA, USA). The NanoString panel can simultaneously quantitate 770 immune-related genes through mRNA quantification. All procedures, including preparation, hybridization, detection, scanning, and norma...
All results, except for clinical samples, are based on at least three independent tests. The results are presented in terms of the mean with standard deviation, and are graphed. We applied ImageJ (RRID:SCR_003070, National Institutes of Health, Bethesda, MD, USA) to quantify the results of Western blots. We performed a paired samples t test or ANOV...
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Clinical Interests. Narcolepsy, Sleep disturbance, Migraine, Numbness and tingling, Neuropathy. Care Philosophy. I believe in the importance of shared decision making and having a clear dialogue and communication between provider and patient.
(134)134 reviews. Telehealth available. Accepting New Patients. Show phone number (303) 329-8998. About. Dr. Thomas Li, MD works in Denver, CO as an Internal Medicine Specialist. He is...
