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Jun 27, 2016 · In the same time frame, advances in cell line engineering, media composition, and bioreactor design led to 10-fold increases in titers for batch and fed-batch modes, eliminating the first driver for adoption of perfusion technology.
Perfusion culture is a technique where modified fermenters of up to 100 dm3 are gently stirred and broth is withdrawn continuously from the vessel and passed through a stainless steel or ceramic filter. This type of culture is sometimes referred to as spin culture, since the filter is spun to prevent blocking with cells.
Nov 25, 2022 · Bioreactors enabling direct perfusion of cell suspensions or culture media through the pores of 3D scaffolds have long been used in tissue engineering to improve cell seeding efficiency as well as uniformity of cell distribution and tissue development.
- 25 November 2022
- 18, Issue2
Jun 29, 2020 · We envision that this protocol will facilitate the adoption and standardization of perfusion tissue culture methods across the fields of biomaterials and tissue engineering.
- Ian S. Kinstlinger, Gisele A. Calderon, Madison K. Royse, A. Kristen Means, Bagrat Grigoryan, Jordan...
- 2021
In this chapter, we give an overview of the challenges and objectives in operating mammalian cell perfusion cultures and provide guidelines for the design and set-up of lab-scale bioreactor systems.
- Moritz Wolf, Jean-Marc Bielser, Massimo Morbidelli
- 2020
Oct 1, 2012 · A tissue engineering approach involves the implantation in the site of injury of a 3D porous biodegradable structure seeded with osteoblastic or mesenchymal stem cells, which are one valuable cell source for orthopedic applications and its growth rate and osteogenic differentiation have been studied for years. 11 The construct would provide a ...
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Jan 1, 2023 · This chapter gives a general overview on the basic requirements for designing bioreactors for tissue engineering. It comprises the general components of a bioreactor and discusses the choice of suitable culture parameters for a tissue engineering process.
