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GUS plc. GUS plc was a FTSE 100 retailing, manufacturing and financial conglomerate based in the United Kingdom. GUS was an abbreviation of Great Universal Stores, the company's name before 2001, while it was also known as the Glorious Gussies amongst stockbrokers. [2] The company started out as Universal Stores, a mail order business created ...
- 1 Kinetic Fluorometric Assay
- 2 Endpoint Fluorometric Assay
- 3 Protein Assay
1. Aliquot approx 2–5 mg (50–200 seeds) of Arabidropsis seeds into a 1.5-mL Eppendorf tube and grind thoroughly in a 150 µL GUS extraction buffer using a motorized micropestle. Remember to assay no...2. Centrifuge the homogenate in an Eppendorf centrifuge, at maximum speed, for 5 min, then place on ice.3. Switch on the Fluoroskan II, as it requires at least 30 min to warm up.4. Aliquot 100 µL of substrate solution into each well. Prepare sufficient wells to assay each sample in duplicate (see Note 7).1. In an Eppendorf tube, mix 100 µL of sample extract with 400 µL of 1 mM4-MUG made up in GUS extraction buffer.2. Place the Eppendorf tube at 37°C. After approx 2 min, remove a 100-µL aliquot of the reaction mix and add this to an equal volume of 0.2MNa2CO3 (stopping reagent). This timepoint is T= 0. Repeat...3. Measure the fluorescence of the stopped reactions, plot against time, and calculate the rate. Note: The highly alkaline stopping reagent results in significant amplification of the fluorescence...1. First prepare a calibration curve using bovine serum albumin (BSA) standards. In 1.6-mL disposable cuvets aliquot 1, 2, 4, 6, 8, 10, and 12 µL of BSA from an aqueous solution of 1 mg/mL. Add 5 µ...2. At the same time, place 5 µL of extract in a 1.6-mL disposable cuvet. Make the volume up to 800 µL with distilled water and add 200 µL of Bradford reagent. Mix by inversion, and leave at room te...3. Standardize samples by expressing GUS activities as pmol 4-MU/pg protein/min (see Note 9).- Gillian A. Hull, Martine Devic
- 1995
Store at 4°C in the dark. Dilute the 100 μ M 4-MU stock solution to 500 n M with 0.2 M sodium carbonate, and prepare serial dilutions to 200, 150, 100, 50, and 10 n M in 0.2 M sodium carbonate buffer.
- Kye-Won Kim, Vincent R. Franceschi, Laurence B. Davin, Norman G. Lewis
- 2006
Apr 2, 2021 · Release date: 1 April 2021. P-line: ℗ Road Story Records. SoundCloud - Hear the world's sounds.
Check out The Dark Side of Light (Original Motion Picture Soundtrack) by Gus Reyes on Amazon Music. Stream ad-free or purchase CD's and MP3s now on Amazon.co.uk.
Cartoon illustration is an art form that allows artists to express their ideas, humour and views through humorous, evocative and satirical images. One visual artist who has created works that have inspired and sparked discussion among many is Gus Dark, a talented visual artist from Bal i. Gus Dark was born in east Bali, Karangasem and since ...
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Feb 14, 2022 · 2 RESULTS. 2.1 Acetone fixation improves GUS staining intensity. During our work, we noticed that GUS staining in dark-grown hypocotyls oftentimes produces patchy staining patterns that did not correspond with the localization patterns seen when using other reporter proteins.
