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1. pubmed.ncbi.nlm.nih.gov › 32310382Enzyme Linked Immunosorbent Assay - PubMed

   pubmed.ncbi.nlm.nih.gov › 32310382

   Enzyme immunoassays (EIAs) use the catalytic properties of enzymes to detect and quantify immunologic reactions. Enzyme-linked immunosorbent assay (ELISA) is a heterogeneous EIA technique used in clinical analyses.

   • Author: Mandy Alhajj, Aisha Farhana

   • Publish Year: 2021

2. pubs.acs.org › doi › 10High-Throughput, High-Multiplex Digital Protein Detection ...

   pubs.acs.org › doi › 10

   Jan 14, 2022 · Multiplexed digital ELISA in picoliter droplets based on enzyme signal amplification block and precisely decoding strategy: A universal and practical biodetection platform. Sensors and Actuators B: Chemical 2022, 369 , 132214. https://doi.org/10.1016/j.snb.2022.132214

3. www.ncbi.nlm.nih.gov › pmc › articlesCorrelation between a quantitative anti‐SARS‐CoV‐2 IgG ELISA ...

   www.ncbi.nlm.nih.gov › pmc › articles

   Aug 31, 2021 · The present study investigated the level of correlation between a novel standardized enzyme‐linked immunosorbent assay (ELISA) for the quantitative detection of anti‐SARS‐CoV‐2 S1 IgG and a microneutralization assay.

   • Author: Ramona Dolscheid-Pommerich, Eva Bartok, Marcel Renn, Beate M Kümmerer, Bianca Schulte, Ricarda M Sch...
   • DOI: 10.1002/jmv.27287

   • Publish Year: 2022
   • Publication: J Med Virol. 2022 Jan; 94(1): 388-392.

4. openres.ersjournals.com › content › 9/4/00746-2022Transition to endemic: 2-year SARS-CoV-2 surveillance follow ...

   openres.ersjournals.com › content › 9/4/00746-2022

   • Cached

   Here, we report the 2-year follow-up results of the ELISA cohort. The primary aim was to analyse potential risk factors during a high-incidence period and the effects of large-scale vaccination programmes on the seroprevalence of antibodies against the spike (S1) antigen.

5. www.science.org › doi › 10One-year surveillance of SARS-CoV-2 transmission of the ELISA ...

   www.science.org › doi › 10

   Apr 15, 2022 · The ELISA study is the first to provide longitudinal data throughout the SARS-CoV-2 pandemic on a prospectively followed, population-based cohort.

6. www.ncbi.nlm.nih.gov › books › NBK555922Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf

   www.ncbi.nlm.nih.gov › books › NBK555922

   Apr 23, 2023 · Enzyme immunoassays (EIAs) use the catalytic properties of enzymes to detect and quantify immunologic reactions. Enzyme-linked immunosorbent assay (ELISA) is a heterogeneous EIA technique used in clinical analyses. [1]

7. People also ask

   What is Elisa & why is it important?

   • A major challenge in many clinical diagnostic applications is the measurement of low-abundance proteins and other biomolecules in biological fluids. Digital technologies such as the digital enzyme-linked immunosorbent assay (ELISA) have enabled 1000-fold increases in sensitivity over conventional protein detection methods.

   High-Throughput, High-Multiplex Digital Protein Detection with Attomo…

   pubs.acs.org/doi/10.1021/acsnano.1c08675

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   Who approved the Elisa study?

   • The ELISA study was approved by the ethics committee of the University of Lübeck (Az. 20-150). Participants were invited to take part in the study through announcements in the local press, posters, and leaflets and by advertisement on the official homepage of the city of Lübeck by its mayor.

   One-year surveillance of SARS-CoV-2 transmission of the ELISA ... - Scie…

   www.science.org/doi/10.1126/sciadv.abm5016

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   How does a direct ELISA work?

   • In a direct ELISA, the primary detection antibody binds directly to the protein of interest. Next, the plate is rewashed to remove any unbound antibodies. An enzyme, such as alkaline phosphatase (AP) or horseradish peroxidase (HRP), is added to the plate, which results in a color change.

   Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf

   www.ncbi.nlm.nih.gov/books/NBK555922/

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   What are ELISA assays used for?

   • In addition, ELISA assays have been used extensively to detect antibodies to viruses and autoantigens in serum or whole blood. In addition, enzyme conjugates coupled with substrates that produce visible products have been used to develop ELISA-type assays with results that can be interpreted visually.

   Enzyme Linked Immunosorbent Assay - PubMed

   pubmed.ncbi.nlm.nih.gov/32310382/

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   What does Elisa stand for?

   • Enzyme-linked immunosorbent assays (ELISA). [Curr Protoc Mol Biol. 2001] Enzyme-linked immunosorbant assay (ELISA). [Methods Mol Biol. 1984] Review Enzyme-linked immunosorbent assays. [Curr Protoc Immunol. 2001] Review Enzyme immunoassay techniques. An overview. [J Immunol Methods. 1992] See reviews... See all... See more...

   Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf

   www.ncbi.nlm.nih.gov/books/NBK555922/

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   What are the advantages and disadvantages of direct ELISA?

   • The advantages of using direct ELISA include eliminating secondary antibody cross-reactivity, and due to fewer steps, it is rapid compared to indirect ELISA. Its disadvantages include its low sensitivity compared to the other types of ELISA and its high cost of reaction. Indirect ELISA

   Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf

   www.ncbi.nlm.nih.gov/books/NBK555922/

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8. pubmed.ncbi.nlm.nih.gov › 34415572Correlation between a quantitative anti-SARS-CoV-2 IgG ELISA ...

   pubmed.ncbi.nlm.nih.gov › 34415572

   Aug 21, 2021 · This study analyzes the level of correlation between the novel quantitative EUROIMMUN Anti-SARS-CoV-2 QuantiVac ELISA (IgG) and a microneutralization assay.