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1. www.nature.com › articles › s41467/020/20383-xStandardization of ELISA protocols for serosurveys of the ...

   www.nature.com › articles › s41467/020/20383-x

   Jan 4, 2021 · Here, we present an optimized ELISA-based serology protocol, from antigen production to data analyses, that helps define thresholds for IgG and IgM seropositivity with high specificities.

   • Author: Carleen Klumpp-Thomas, Heather Kalish, Matthew Drew, Sally Hunsberger, Kelly Snead, Michael P. Fay, ...

   • Publish Year: 2021

2. pubmed.ncbi.nlm.nih.gov › 33397956Standardization of ELISA protocols for serosurveys of the ...

   pubmed.ncbi.nlm.nih.gov › 33397956

   Jan 4, 2021 · High quality serology is key to avoiding medically costly diagnostic errors, as well as to assuring properly informed public health decisions. Here, we present an optimized ELISA-based serology protocol, from antigen production to data analyses, that helps define thresholds for IgG and IgM seropositivity with high specificities.

   • Author: Carleen Klumpp-Thomas, Heather Kalish, Matthew Drew, Sally Hunsberger, Kelly Snead, Michael P. Fay, ...

   • Publish Year: 2021

3. pubmed.ncbi.nlm.nih.gov › 32310382Enzyme Linked Immunosorbent Assay - PubMed

   pubmed.ncbi.nlm.nih.gov › 32310382

   Enzyme immunoassays (EIAs) use the catalytic properties of enzymes to detect and quantify immunologic reactions. Enzyme-linked immunosorbent assay (ELISA) is a heterogeneous EIA technique used in clinical analyses.

   • Author: Mandy Alhajj, Aisha Farhana

   • Publish Year: 2021

4. www.nature.com › articles › s41586/021/03207-wEvolution of antibody immunity to SARS-CoV-2 - Nature

   www.nature.com › articles › s41586/021/03207-w

   Jan 18, 2021 · Antibody reactivity in plasma to the RBD and nucleoprotein (N) of SARS-CoV-2 was measured by enzyme-linked immunosorbent assay (ELISA) and automated serological assays 1,5,6.

   • Author: Christian Gaebler, Zijun Wang, Julio C. C. Lorenzi, Frauke Muecksch, Shlomo Finkin, Minami Tokuyama,...

   • Publish Year: 2021

5. www.ncbi.nlm.nih.gov › books › NBK555922Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf

   www.ncbi.nlm.nih.gov › books › NBK555922

   Apr 23, 2023 · Enzyme immunoassays (EIAs) use the catalytic properties of enzymes to detect and quantify immunologic reactions. Enzyme-linked immunosorbent assay (ELISA) is a heterogeneous EIA technique used in clinical analyses. [1]

   • Author: Mandy Alhajj, Aisha Farhana
   • Published: 2023/04/23

   • Publish Year: 2021

6. www.ncbi.nlm.nih.gov › pmc › articlesEnzyme-Linked Immunosorbent Assay: An Adaptable Methodology ...

   www.ncbi.nlm.nih.gov › pmc › articles

   Mar 9, 2022 · ELISA can be modified to assess the functionality of antibodies, as avidity and neutralization, respectively by the standardization of avidity-ELISA and surrogate-neutralization methods. Cellular immunity can also be studied using this assay.

7. People also ask

   What is ELISA based serology assay protocol?

   • Here, we present an optimized enzyme-linked immunosorbent assays (ELISA)-based serology assay protocol—from protein production to data analysis—that analyzes the presence of IgG, IgM, and IgA antibodies against spike and RBD antigens of SARS-CoV-2.

   Standardization of ELISA protocols for serosurveys of the SARS ... - Nature

   www.nature.com/articles/s41467-020-20383-x

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   How can Elisa be modified?

   • ELISA can be modified to assess the functionality of antibodies, as avidity and neutralization, respectively by the standardization of avidity-ELISA and surrogate-neutralization methods. Cellular immunity can also be studied using this assay.

   Enzyme-Linked Immunosorbent Assay: An Adaptable Methodology to S…

   www.ncbi.nlm.nih.gov/pmc/articles/PMC8948777/

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   Is Elisa a good method for HCP quantitation?

   • The enzyme-linked immunosorbent assay (ELISA) is still considered the gold standard for HCP measurement, having advantages like high speed, sensitivity, and high throughput. Nevertheless, to overcome intrinsic limitations of individual methods for HCP quantitation, the implementation of orthogonal methods is strongly advised (10).

   Making the Right Choices for Reliable HCP Monitoring by ELISA

   www.biogenes.de/fileadmin/user_upload/Dokumente/BioGenes_Editorial_EBR_Winter_2021_HCP_Monitoring.pdf

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   Is Elisa still relevant?

   • ELISA and ELISA-based methods aided the area of immunology against infectious diseases and is still relevant, for example, as a promising approach to study the differences between natural and vaccine-induced immune responses against SARS-CoV-2. 1. Introduction

   Enzyme-Linked Immunosorbent Assay: An Adaptable Methodology to S…

   www.ncbi.nlm.nih.gov/pmc/articles/PMC8948777/

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   Does a combination ELISA assay provide optimal results for specificity?

   • Through careful evaluation of various ELISA assays and statistical determination of optimal threshold cutoffs for specificity, we determined that a combination approach using two ELISA assays, one employing the McLellan/VRC spike construct and the other employing the Ragon RBD, provided optimal results (Fig. 6).

   Standardization of ELISA protocols for serosurveys of the SARS ... - Nature

   www.nature.com/articles/s41467-020-20383-x

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   Why is ELISA based serology important?

   • High quality serology is key to avoiding medically costly diagnostic errors, as well as to assuring properly informed public health decisions. Here, we present an optimized ELISA-based serology protocol, from antigen production to data analyses, that helps define thresholds for IgG and IgM seropositivity with high specificities.

   Standardization of ELISA protocols for serosurveys of the SARS ... - Nature

   www.nature.com/articles/s41467-020-20383-x

   See all results for this question

8. www.biogenes.de › fileadmin › user_uploadMaking the Right Choices for Reliable HCP Monitoring by ELISA

   www.biogenes.de › fileadmin › user_upload

   The enzyme-linked immunosorbent assay (ELISA) is still considered the gold standard for HCP measurement, having advantages like high speed, sensitivity, and high throughput. 60 Martin Föge and Stefan Sommerschuh at BioGenes European Biopharmaceutical Review | January 2021