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Resolution. Resolution is the ability to distinguish between two separate points. If two separate points cannot be resolved, they will be observed as one point. The resolution of a light microscope is limited by the wavelength of light. As light passes through the specimen, it will be diffracted. The longer the wavelength of light, the more it ...
Describe a wet mount sample. When the specimens are added to water before the coverslip is lowered with a mounted needle to prevent bubbles forming eg aquatic organisms. Describe a squash slide sample. Wet mounts which are then pressed down to ensure a thin layer to allow light to be passed through eg viewing chromosomes in mitosis.
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Magnification refers to the increase in size of an object when viewed through a microscope, while resolution refers to the ability of a microscope to distinguish between two separate objects that are close together. The higher the resolution of a microscope, the better it is able to separate objects that are close together and to provide clear ...
Resolution is the ability to distinguish between two separate points (or how detailed the image is). For example, both images here have the same magnification, but the left image has a higher resolution. - How large the object appears when you view it through a microscope. When calculating magnification, all lengths must be in the same unit.
Worked example. Step 1: Convert all units to µm. 1 mm = 1000 µm, so converting mm to µm involves multiplying by 1000. 50 × 1000 = 50 000. The a ctual thickness of the leaf is 2000 µm and the i mage size is 50 000 µm. Step 2: Calculate magnification using the formula. magnification = image size ÷ actual size. = 50 000 / 2000.
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To calculate magnification, you need to know the image size and the object size: Image size - How large the object appears when you view it through a microscope. magnification = image size object size \text {magnification }=\frac {\text {image size}} {\text {object size}} . When calculating magnification, all lengths must be in the same unit ...
