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Resolution is the ability to distinguish between two separate points. If two separate points cannot be resolved, they will be observed as one point. The resolution of a light microscope is limited by the wavelength of light. As light passes through the specimen, it will be diffracted. The longer the wavelength of light, the more it is ...
State how to calibrate an eyepiece graticulate. 1) Look through the eyepiece and line up the stage micrometre and eyepiece graticulate 2) Count how many divisions on the eyepiece graticulate fit one division on micrometer scale 3) Each division on micrometer is 10um. What is a stage micro meter and what is its measuring scale.
The most important property of a microscope is its resolution close resolution The fineness of detail that can be seen in an image - the higher the resolution of an image, the more detail it holds.
Describe a wet mount sample. When the specimens are added to water before the coverslip is lowered with a mounted needle to prevent bubbles forming eg aquatic organisms. Describe a squash slide sample. Wet mounts which are then pressed down to ensure a thin layer to allow light to be passed through eg viewing chromosomes in mitosis.
Magnification refers to the increase in size of an object when viewed through a microscope, while resolution refers to the ability of a microscope to distinguish between two separate objects that are close together. The higher the resolution of a microscope, the better it is able to separate objects that are close together and to provide clear ...
To calculate magnification, you need to know the image size and the object size: Image size - How large the object appears when you view it through a microscope. magnification = image size object size \text {magnification }=\frac {\text {image size}} {\text {object size}} . When calculating magnification, all lengths must be in the same unit ...
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Dec 24, 2022 · ” The term magnification is often confused with the term “resolution,” which describes the ability of an imaging system to show detail in the object that is being imaged. While high magnification without high resolution may make very small microbes visible, it will not allow the observer to distinguish between microbes or sub-cellular parts of a microbe.
