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Resolution. Resolution is the ability to distinguish between two separate points. If two separate points cannot be resolved, they will be observed as one point. The resolution of a light microscope is limited by the wavelength of light. As light passes through the specimen, it will be diffracted. The longer the wavelength of light, the more it ...
Dec 24, 2022 · ” The term magnification is often confused with the term “resolution,” which describes the ability of an imaging system to show detail in the object that is being imaged. While high magnification without high resolution may make very small microbes visible, it will not allow the observer to distinguish between microbes or sub-cellular parts of a microbe.
Topic 2 - Cells. Active immunity: A form of immunity provided by the immune response of the body upon detection of a pathogen. Active transport: The active movement of substances from a low concentration to a higher concentration (up their concentration gradient) with the use of energy in the form of ATP. Agglutination: The clumping together of ...
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Resolution is the ability to distinguish between two separate points (or how detailed the image is). For example, both images here have the same magnification, but the left image has a higher resolution. To calculate magnification, you need to know the image size and the object size: Image size - How large the object appears when you view it ...
Magnification refers to the increase in size of an object when viewed through a microscope, while resolution refers to the ability of a microscope to distinguish between two separate objects that are close together. The higher the resolution of a microscope, the better it is able to separate objects that are close together and to provide clear ...
Worked example. Step 1: Convert all units to µm. 1 mm = 1000 µm, so converting mm to µm involves multiplying by 1000. 50 × 1000 = 50 000. The a ctual thickness of the leaf is 2000 µm and the i mage size is 50 000 µm. Step 2: Calculate magnification using the formula. magnification = image size ÷ actual size. = 50 000 / 2000.
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How can axial resolution be defined?
fl. function of the defocus distance (e.g., the distance above and below the focal plane). The axial resolution (along the optical axis) can then be de fined as the width of the plot at half-maximum intensity. Optical aberrations in the microscope cause a strong asymmetry in the plot of light intensity versus distance.
