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Laser sources, despite the high cost, have become especially useful in laser scanning confocal microscopy. Explore how a ruby laser is excited by a xenon flash tube to produce coherent red light. There are a number of different types of lasers that each provide a unique emission spectrum.
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Homogeneity of the light source is the important aspect when considering this method of illumination. The flame produced by a burning lamp is fairly even and consistent, but other sources such as frosted enlarger bulbs, opal bulbs, or ribbon filaments can also be used for critical illumination.
It is possible to remove the image of the filament from the field of view by using an opal light bulb, or a fine-grain diffuse glass screen in front of a clear bulb.
Nov 13, 2015 · The most common source for today's microscopes is an incandescent tungsten-halogen bulb positioned in a reflective housing that projects light through the collector lens and into the substage condenser. Other sources include arc-discharge lamps, light emitting diodes (LEDs), and lasers.
Flash tubes provide 5,500 K illumination in an instantaneous burst that can capture a significant amount of object detail for spectacular results in photography, digital imaging, and photomicrography.
Light microscopes commonly used college classroom laboratories are used to magnify specimens up 400 times (400x), but oil immersion lenses can also be used with these microscopes for magnification of 1,000 times (1000x) to view very small structures or very small cells such as bacteria.
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May 29, 2020 · 2.2.1.3 Flash. A flash can be used for taking photomicrographs of motile specimens. Thus, any indistinctness caused by locomotion can be avoided. The color temperature of a flash is circa 6500 K so that a slight bluish hue may be visible. This dominance of blue can be mitigated by appropriate filters.