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A bromide ion is the negatively charged form (Br−) of the element bromine, a member of the halogens group on the periodic table. Most bromides are colorless. Bromides have many practical roles, being found in anticonvulsants, flame-retardant materials, and cell stains. [3]
Revision notes on 1.3.3 Protein Structure & Function for the AQA A Level Biology syllabus, written by the Biology experts at Save My Exams.
Ethidium Bromide. Ethidium bromide (Ethidium bromide=2,7-Diamino-10-ethyl-9-phenyl-phenanthridinium bromide) (EtBr) remains a widely used dye for the visualization of nucleic acids in agarose gels. It is a phenanthridinium intercalator, structurally similar to propidium iodide.
Ethidium bromide (or homidium bromide, [2] chloride salt homidium chloride) [3] [4] is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Apr 4, 2021 · Chemical structure of ethidium bromide, IUPAC name: 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide. During the early 1960s, experimental and spectroscopic work showed direct evidence of a strong interaction between planar acridine derivatives (e.g. proflavine, daunorubicin etc.) and nucleic acids which led to speculation about the ...
- Rodrigo Galindo-Murillo, Thomas E Cheatham
- 10.1093/nar/gkab143
- 2021
- 2021/04/04
Mar 21, 2024 · Chemical reagents, such as cyanogen bromide, which cleaves peptide bonds on the C-terminal side of a methionine residue can also be used to cut larger proteins into smaller peptides. Common proteins performing this activity are found in the digestive system and are shown below.
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Ethidium Bromide is a dye that shows significant fluorescence enhancement when it binds to double-stranded DNA by intercalating between adjacent base pairs. It is commonly used as a stain in various molecular biology techniques due to its efficient penetration of electrophoretic gels.
