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Nov 3, 2023 · Agglutination is the visible expression of the aggregation of antigens and antibodies. Agglutination reactions apply to particulate test antigens that have been conjugated to a carrier. The carrier could be artificial (such as latex or charcoal particles) or biological (such as red blood cells).
Agglutination is clumping together in suspension of cells bearing the antigen (epitopes)/ antigen bearing microorganisms, or particles in the presence of specific antibodies called “agglutinins”.
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Apr 10, 2024 · Agglutination reaction is an antigen-antibody reaction that leads to the visible clumping of particles. It occurs when a particulate antigen combines with its corresponding antibody in the presence of electrolytes, at a specific temperature and pH.
The anti globulin reagent is prepared by immunizing animals, often rabbits with human gamma globulin (antibody) and, or beta globulin (components of complement). There are two types of anti globulin reagents that can be used in laboratory procedure: broad spectrum (polyspecific sera) and monospecfic sera.
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Nov 14, 2023 · Agglutination can be used as an indicator of the presence of antibodies against bacteria or red blood cells. Agglutination assays are usually quick and easy to perform on a glass slide or microtiter plate (Figure 50.6.3.1 50.6.3. 1).
Feb 28, 2023 · The purpose of agglutination is to detect the presence of specific antigens or antibodies in samples of bodily fluids, such as blood, saliva, urine, or cerebrospinal fluid (CSF); as well as to identify specific strains of bacteria in microbiological cultures.
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Learning Objectives. Compare direct and indirect agglutination. Identify various uses of hemagglutination in the diagnosis of disease. Explain how blood types are determined. Explain the steps used to cross-match blood to be used in a transfusion.