Search results
Agglutination is one of the antigen and specific antibody reactions in-vitro in laboratory in the presence of electrolytes at a suitable temperature and pH. Agglutination is used for diagnosis of diseases in laboratory either uses the particulate or soluble antigens. Agglutination involves two steps.
- 282KB
- 20
Apr 10, 2024 · Agglutination reaction is an antigen-antibody reaction that leads to the visible clumping of particles. It occurs when a particulate antigen combines with its corresponding antibody in the presence of electrolytes, at a specific temperature and pH.
- What Is agglutination?
- Prozone Phenomenon
- Types of Agglutination Reactions
- Active Agglutination
- Passive Agglutination
- Hemagglutination Test
- Applications of Agglutination Reactions
- Further Readings
Agglutination is the visible expression of the aggregation of antigens and antibodies. Agglutination reactions apply to particulate test antigens that have been conjugated to a carrier. The carrier could be artificial (such as latex or charcoal particles) or biological (such as red blood cells). These conjugated particles are reacted with patient s...
The condition of excess antibody, however, is called a prozone phenomenon.At a high concentration of antibody, the number of epitopes are outnumbered by antigen-binding sites. This results in the univalent binding of antigen by antibody rather than multivalently and thus, interferes in the crosslinking of antigen (Lattice formation). Occasionally, ...
Agglutination reactions can be broadly divided into three groups: 1. Active/Direct agglutination 2. Passive agglutination 3. Hemagglutination
Agglutination reactions where the antigens are found naturally on a particle are known as direct agglutination. In active agglutination, direct agglutination of particulate antigen with specific antibody occurs. Direct bacterial agglutination uses whole pathogens as a source of antigen. It measures the antibody level produced by a host infected wit...
Passive agglutination employs carrier particles that are coated with soluble antigens. In this either antibody or antigen is attached to certain inert carrier thereby, particles or cells gets agglutinated when corresponding antigen or antibody reacts. Latex particles, Carbon particles, Bantonite etc. are used as inert carriers. E.g. Antigens coated...
RBCs are used as carrier particles in hemagglutination tests. RBCs of sheep, human, chick, etc. are commonly used in the test. When RBCs are coated with antigen to detect antibodies in the serum, the test is called indirect hemagglutination (IHA) test. Hemagglutination uses erythrocytes as the biological carriers of bacterial antigens, and purified...
Cross-matching and grouping of blood.Identification of Bacteria. E.g. Serotyping of Vibrio cholera, Serotyping of SalmonellaTyphi and Paratyphi.Serological diagnosis of various diseases. E.g Rapid plasma regains (RPR) test for Syphilis, Antistreptolysin O (ASO)test for rheumatic fever.Detection of unknown antigen in various clinical specimens. E.g. detection of Vi antigen of SalmonellaTyphi in the urine.https://bio.libretexts.org/Bookshelves/Microbiology/Book%3A_Microbiology_(Boundless)/12%3A_Immunology_Applications/12.2%3A_Immunoassays_for_Disease/12.2E%3A__Agglutination_Reactionshttps://www.brainkart.com/article/Types-of-Agglutination-Reactions—Antigen-Antibody-Reactions_20188/http://www.biosciencenotes.com/agglutination/Nov 14, 2023 · Agglutination can be used as an indicator of the presence of antibodies against bacteria or red blood cells. Agglutination assays are usually quick and easy to perform on a glass slide or microtiter plate (Figure 50.6.3.1 50.6.3. 1).
Classification of the blood group was based on his observation of the agglutination reaction between an antigen on erythrocytes and antibodies present in the serum of individuals directed against these antigens. Where no agglutination had occurred, either the antigen or the antibody was missing from the mixture.
- 650KB
- 126
Immunology and serology is an advanced science dealing with how the human immune system organized, function and the different types of serological techniques. It is a very vast subject covering a wide area of technology.
People also ask
What is the purpose of agglutination?
What is active/direct agglutination?
How are agglutination results interpreted?
What is agglutination test?
What is agglutination reaction?
What is the process of agglutination?
Agglutination is caused by the formation of antibody-antigen complexes and occurs at room temperatures. Auto-agglutination is produced as a result of a complex formed between the patient’s own RBC antigens and antibodies, mediated by cold-reacting antibodies.
Download wonderful eBooks & Audiobooks now - for Free
