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Jan 1, 2014 · The agglutination phenomena can be defined as the linkage of particles or cells in a liquid resulting in formation of clumps. In detail, aggregation/agglutination is the outcome of...
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- Ring Precipitation Test
- Gel Diffusion Precipitation Test
- Single Diffusion
- Double Diffusion
The ring precipitation test is usually performed in a tube, and requires the formation of antigen-–antibody precipitate between two fluids as a ring of precipitate that is layered on the surface of the antibody (Fig. 3.2) [1, 2].
In gel diffusion precipitation tests, antibodies and antigens diffuse together in agar medium forming a line of precipitate . This test is available in two principles, single diffusion and double diffusion .
In single diffusion, the antibodies are spread homogeneously in agar medium and the antigens diffuse within the agar . Here, the gel agar contains diluted antibodies specific to certain antigens . A ring of precipitate forms upon the addition of the antigens into the well, which is a cut in the agar . The ring forms at the equivalent point between...
In double diffusion assays, antibodies and antigens are applied into two different wells in an agar medium and allowed to diffuse toward each other, forming a thin line of precipitate that meets at their equivalent point . The concentration depends on the position of the line and the diffusion co-efficient of both the antibodies and the antigens (F...
- Rowa Yousef Alhabbab
- 2018
Oct 13, 2015 · Influenza hemagglutinin (HA) is a virus surface glycoprotein that readily binds to sialic acid receptors on the membranes of red blood cells (RBCs). Sufficient quantities of HA will cause RBCs to clump together, or agglutinate. However, RBC binding is inhibited when influenza antibodies bind to antigenic sites on the HA molecule.
- Michael Nguyen, Robert Parkhill
- 2015
Agglutination occurs when an insoluble or particle antigen interacts with an antibody. A positive reaction can be detected macroscopically in a short time. However, the antigen-antibody complex may be seen with the naked eye if the complex size is large. Both IgG or IgM could be involved in the agglutination reaction.
Jul 30, 2023 · Definition of Agglutination: Agglutination is the process of particles clumping together due to specific interactions between antigens and antibodies. Types of Agglutination: There are several types of agglutination, each with its own specific context and applications. Here are some common types:
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May 20, 2020 · In Harvard style, to reference a journal article, you need the author name(s), the year, the article title, the journal name, the volume and issue numbers, and the page range on which the article appears. If you accessed the article online, add a DOI (digital object identifier) if available.
