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1. • • Click on one of the cells within the data set you just entered and then click the Charts Tab below the toolbar. By clicking in the data set, you have identified what values are to be used in generating the graph. A Chart Ribbon will open. Choose the XY (Scatter) button.
     Reference:

     Excel tutorial - St. Mary's College of Maryland

2. People also ask

   How do you calculate protein concentration based on absorbance?

   • Plotting a graph with the absorbance value as the dependent variable (Y-axis) and concentration as the independent variable (X-axis), results in an equation formatted as follows: y = ax2 + bx + c, where solving for x determines the protein concentration of the sample.

   How to use a protein assay standard curve - Thermo Fisher Scientific

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   How to create a standard curve in Excel?

   • To create a standard curve in Microsoft Excel, two data variables are required. One set of data must be the independent variable, which is the known values (e.g. protein standard concentrations in a BCA assay), and the other is the dependent variable which refers to the measured values (e.g. the optical density readings of the samples).

   How To Create A Linear Standard Curve In Excel - Top Tip Bio

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   How much protein is in a test sample?

   • Because 10μL of the standard sample is added to a well, there is 0.010mL × 1000μg/mL = 10μg of protein in the well. If the assay results in the test sample having the same final absorbance as the standard sample, then the conclusion is that the test sample contains the same amount of protein as the standard sample.

   How to use a protein assay standard curve - Thermo Fisher Scientific

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   How does a protein assay work?

   • Typical protein assays are used to determine protein concentration by comparing the assay response of a sample to that of a standard whose concentration is known. Protein samples and protein standards are processed in the same manner by mixing them with assay reagent and using a spectrophotometer to measure the absorbances.

   How to use a protein assay standard curve - Thermo Fisher Scientific

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   How much protein is in a microplate reagent?

   • (Ordinarily, an entire set of standards is necessary to establish a response curve, but this is a simplified example.) In the microplate protocol, one adds 10μL of sample (test or standard) and 300μL of assay reagent per well. Because 10μL of the standard sample is added to a well, there is 0.010mL × 1000μg/mL = 10μg of protein in the well.

   How to use a protein assay standard curve - Thermo Fisher Scientific

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   How to create a scatter plot in Excel?

   • Select the two columns in Excel. To select more than one column in Excel, hold down the ‘ ctrl ‘ key while you click and drag the mouse over the data. Then go to, ‘ Insert > Scatter ‘ and select the first (‘ Scatter ‘) option. 2. Hopefully a scatter plot will now be displayed.

   How To Create A Linear Standard Curve In Excel - Top Tip Bio

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3. Videos

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     How to calculate Protein Concentration of Unknown Sample from standard curve in excel

     23 Sep 2020

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     How to Calculate Protein Concentration of Unknown Sample from Standard Curve in Excel

     19 Apr 2023

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     3:17

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     Generating Standard Curve and Determination of Protein Concentration in Excel

     30 Sep 2021

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4. toptipbio.com › linear-standardHow To Create A Linear Standard Curve In Excel - Top Tip Bio

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   How To Create A Linear Standard Curve In Excel. In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown sample values. I will use the BCA total protein assay standards as an example.

5. www.exceldemy.com › how-to-determine-proteinHow to Determine the Protein Concentration from a Standard ...

   www.exceldemy.com › how-to-determine-protein

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   Aug 13, 2024 · This article shows 2 easy ways to Determine Protein Concentration from Standard Curve in Excel. Learn them, download workbook and practice.

6. assets.thermofisher.com › TFS-Assets › LSGHow to use a protein assay standard curve

   assets.thermofisher.com › TFS-Assets › LSG

   Using Microsoft Excel to plot and apply standard curve A protein assay, such the BCA Protein Assay, is an excellent tool for estimating the protein concentration of a sample. The intensity of the colored reaction product is a direct function of protein amount that can be determined by comparing its absorbance value to a standard curve.

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7. www.youtube.com › watchHow to calculate Protein Concentration of Unknown Sample from ...

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   This video explains about How to calculate Protein Concentration of Unknown Sample from standard curve in excel Simple method for the Determination of unknown protein concentration from...

   • Video Duration: 3 min
   • Views: 262.1K

   • Author: Bio-Resource

8. www.youtube.com › watchHow to Calculate Protein Concentration of Unknown Sample from ...

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   In this video, we demonstrate step-by-step how to calculate the protein concentration of unknown samples using a standard curve prepared using absorbance and concentration in Excel. We use the...

   • Video Duration: 7 min
   • Views: 19.6K

   • Author: Biology Lectures

9. www.youtube.com › watchBradford Assay - Using Microsoft Excel to Plot a Standard Curve

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   This video describes how to easily use Microsoft Excel to calculate protein concentrations of samples in a Bradford assay.

   • Video Duration: 23 min
   • Views: 7.6K

   • Author: Gary O'Mealey, PhD

10. dashboardsexcel.com › blogs › blogExcel Tutorial: How To Plot Standard Curve In Excel

    dashboardsexcel.com › blogs › blog

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    When working with standard curves in Excel, creating a scatterplot is essential for visualizing the relationship between the variables. This guide will walk you through the steps to create a standard curve scatterplot in Excel. A. Demonstrate how to select the data for the standard curve plot.