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Loading control
- In Western blotting, they are run as a "loading control" to show that very similar amounts of protein were loaded in each lane.
biology.stackexchange.com/questions/40139/why-is-beta-actin-commonly-used-as-a-control-in-western-blotsWhy is beta actin commonly used as a control in Western blots?
People also ask
Can -actin be used as a loading control for Western blot analysis?
Are all Western blot loading control proteins expressed equally?
What is a good loading control for Western blot analysis?
What is Western blot analysis?
Why is Western blot important?
Is beta-actin a reliable loading control in western blot analysis?
Loading controls. Loading controls are proteins that exhibit high-level, constitutive expression in the cell type or sample that you are studying. Housekeeping genes, such as actin, GAPDH, tubulin, or mitochondrial proteins, are usually good choices.
- Abcam
By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This ...
- Tahrin Mahmood, Ping-Chang Yang
- 10.4103/1947-2714.100998
- 2012
- N Am J Med Sci. 2012 Sep; 4(9): 429-434.
- α-Tubulin. Antibodies for western blot with mouse reactivity: - α-Tubulin (11H10) Rabbit mAb #2125. - α-Tubulin Antibody #2144. - α-Tubulin (DM1A) Mouse mAb #3873.
- COX IV. Antibodies for western blot with mouse reactivity: - COX IV Antibody #4844. - COX IV (4D11-B3-E8) Mouse mAb #11967. - COX IV (D6I4K) Rabbit mAb (Rodent Specific) #38563.
- β-Tubulin. Antibodies for western blot with mouse reactivity: - β-Tubulin (9F3) Rabbit mAb #2128. - β-Tubulin Antibody #2146. - β-Tubulin (D2N5G) Rabbit mAb #15115.
- Histone H3. Antibodies for western blot with mouse reactivity: - Histone H3 (96C10) Mouse mAb #3638. - Histone H3 (D1H2) XP® Rabbit mAb #4499. - Histone H3 Antibody #9715.
Aug 15, 2012 · On account of its relatively high and constant expression, β-actin has been widely employed in Western blot of cell cultures and tissue extracts. However, β-actin's presence in human plasma and this protein's putative role as a plasma-based loading control for Western blot analysis remain unknown.
- Rufang Zhang, Deyu Yang, Chanjuan Zhou, Ke Cheng, Zhao Liu, Liang Chen, Liang Fang, Peng Xie
- 2012
Sep 12, 2017 · Abstract. An appropriate loading control is critical for Western blot analysis. Housekeeping proteins (HKPs), such as β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and β-tubulin, are commonly used to normalize protein expression.
In most cases, however, β-actin is overloaded, which results in a failure to detect differences in protein loading. In this study, we attempted to optimize the amount of protein loaded for β-actin detection to permit suitable Western blot analysis data normalization.
