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5 days ago · Nitrocellulose (NC) finds widespread use in propellants and launchers. Nanoscale modification can effectively enhance its combustion performance by shortening the mass and heat transfer distance. However, it also presents greater safety challenges for storage and application.
1 day ago · Consistent with our laboratory procedures, slides were deparaffinized, dehydrated, and hematoxylin stained prior to microdissection. LCM caps were lysed with extraction buffer, obtained lysates were then printed onto nitrocellulose slides and stained with a selected group of antibodies for comparison purposes.
3 days ago · Proteins were transferred to nitrocellulose membrane using iBlot 2 Gel Transfer Device (Invitrogen). Membranes were blocked for 1 hour in 5% milk in PBS-T and incubated overnight with primary antibodies, then for 1 hour with secondary antibodies and dyes (1:5000) before imaging on the Licor (Odyssey DLx).
1 day ago · Comprehending Industrial Nitrocellulose Nitrocellulose: What Is It? Nitrocellulose is a highly flammable compound created by nitrating cellulose with a mixture of nitric and sulfuric acids. Known for its excellent film-forming properties, nitrocellulose is widely used in coatings, inks, and adhesives.
1 day ago · The nitrocellulose membrane was then probed with primary antibodies overnight at 4 °C, followed by incubation with secondary antibodies for 1 h at room temperature. Western blot bands were scanned and analyzed using a fully automated chemiluminescence imaging system, with band density normalized to β-actin content.
3 days ago · After transferring the proteins to nitrocellulose filter membranes and blocking nonspecific binding sites, the membrane was incubated overnight with anti-ZBP1 (1:1000, 13285-1-AP, Proteintech, China) and anti-GAPDH (1:1000, ab8245, Abcam, UK) antibodies at 4°C, followed by incubation with a secondary antibody for 1 h at room temperature.
2 days ago · Proteins were separated on a 10% sodium dodecyl sulfate–polyacrylamide gel and transferred onto a nitrocellulose (NC) membrane (Millipore, Billerica, MA). The NC membrane was first blocked with 5% bovine serum albumin (BioSharp, Anhui, China) for 1 h at 24 °C, followed by incubation with primary antibodies for 24 h at 4 °C, and then with secondary antibodies for 2 h at room temperature.
